复方仙草颗粒对IgA肾病小鼠IL-13及IL-17的影响

Influence of Complex Xiancao Granule on IL-13,IL-17 of IgAN rats

目的:观察复方仙草颗粒对IgA肾病(IgAN)小鼠的影响,探讨复方仙草颗粒对IgAN的治疗机制。方法:采用口服加皮下注射牛血清白蛋白,并联合应用葡萄球菌肠毒素B尾静脉注射的方法,建立小鼠IgAN模型,随机分为5组,给予复方仙草颗粒和强的松片进行治疗,以ELISA法检测各组小鼠血清及肾脏组织中IL-13、IL-17的含量变化。结果:与正常组比较,IgAN小鼠血清及肾脏组织中IL-13、IL-17水平均显著性升高(P<0.01);与对照组比较,复方仙草颗粒高、中剂量组均能显著降低IgAN模型小鼠血清及肾脏组织中IL-13、IL-17水平(P<0.05,P<0.01)。结论:复方仙草颗粒可显著抑制IgAN模型血清及肾脏组织中IL-13、IL-17的分泌,从而达到抑制IgAN炎症反应、抗系膜细胞增殖的作用。

Objective： To observe the effects of Complex Xiancao Granule on the IgAN rats,and it＇s mechanism for the treatment of IgAN.Methods： Oral plus subcutaneous injection of bovine serum albumin,and the combination of staphylococcal enterotoxin B tail vein injection method,the rats model of IgAN were randomly divided into 5 groups to give Complex Xiancao Granule and prednisone tablets for treatment,to enzyme linked immunosorbent assay（ELISA） to detect the serum and kidney tissue of IL-13,IL-17 of the content.Results： Compared with normal group： IgAN serum and kidney tissue of IL-13,IL-17 were significantly higher（P0.01）;compared with model group： high dose of compound of immortality particles can significantly reduce the IgAN Serum and kidney tissue of mice in the IL-13,IL-17 levels（P0.05,P0.01）;compound of immortality was smaller dose group and prednisone group did not significantly reduce the IgAN serum and kidney tissue of mice in the IL-13,IL-17 levels.Conclusion： Complex Xiancao Granule could significantly inhibit the serum IgAN and kidney tissue of IL-13,IL-17 secretion,and thus inhibit inflammation in IgAN,anti-mesangial cell proliferation.