苦参碱调节Bax和Bcl-2蛋白表达诱导肺腺癌A549细胞凋亡的机制

Effect of matrine in regulating the expression of Bax and Bcl-2 in A549 cells which induce apoptosis

目的:探讨Bax和Bcl-2蛋白在苦参碱诱导A549细胞凋亡中的作用。方法:MTT法检测苦参碱对A549细胞生长的抑制作用,流式细胞仪检测细胞凋亡及Bcl-2、Bax和Caspase-3蛋白的表达情况。结果:不同浓度的苦参碱对肺腺癌细胞生长均有抑制作用,抑制率与浓度呈正相关。苦参碱随时间进行性降低Bcl-2蛋白的表达,相应地稳步提高Bax蛋白的表达,同时促进Bax从胞浆内向线粒体移位,紧接着降解Caspase-3蛋白。结论:苦参碱通过调节细胞内Bax和Bcl-2蛋白的表达经线粒体信号转导途径诱导A549细胞的凋亡。

Objective： To investigate the effect of matrine on lung adenocarcinoma and explore the underlying mechanism.Methods： The inhibitory effects of matrine on the growth of A549 cells were tested by using MTT method,apoptosis and the expression of Bcl-2,Bax and Caspase-3 protein was detected by flow cytometry.Results： Matrine with different concentrations had inhibitory effects on the growth of lung adenocarcinoma cells in a concentration-dependent manner.The expression levels of the anti-apoptotic Bcl-2 in matrine-treated cells were decreased in a time-dependent manner.This reduction was coupled to a stable increased expression level of the pro-apoptotic Bax.While the Bax was shifted from the cytosol to the mitochondria in cells treated with matrine time-dependently,followed by cleavage of Caspase-3.Conclusion： Matrine causes death of A549 cells via the mitochondrial apoptotic pathway.These effects are most likely dependent on the regulation of the levels of antiapoptotic and proapoptotic proteins.