生化汤对药物流产后阴道出血作用机制的实验研究

Mechanism of Shenghua Decotion in treating bleeding after medical abortion

目的:探讨生化汤对药物流产后阴道出血的作用及其机理。方法:将怀孕Wistar大鼠随机分为正常组、模型组、中药对照组(益母草颗粒冲剂)、生化汤低、中、高剂量组(4、8、16g/kg)。除正常组外,每组在第7天上午8:00按8.3mg/kg体质量灌胃米非司酮,下午18:00按100mg/kg体质量灌胃米索前列醇,给药后分别对各组TUNEL检测凋亡细胞、ELISA测定大鼠血清TNF-α含量。结果:生化汤各组凋亡细胞数显著增高(P<0.01),血清TNF-α含量显著升高(P<0.01),且呈量效关系,中、高剂量组较中药对照组疗效显著(P<0.05)。结论:TNF-α是生化汤促进细胞凋亡治疗药物流产后阴道出血时间延长的机制之一。

Objective： To investigate the effect and mechanism of Shenghua Decotion in preventing bleeding after medical abortion.Methods： Use drug to make pregnant Wistar rats incomplete abortion,and randomly divide them into normal group,model group,motherwort group,Shenghua Decotion,low,medium and high dose group（4,8,16g/kg）.Detect apoptosis in each group by TUNEL,and serum TNF-α levels by ELISA,separately.Results： In Shenghua Decotion groups,apoptotic cells was significantly increased（P0.01）,serum TNF-α was significantly increased（P0.01）,and dose-effect relationship was showed.The medium and high-dose group had better efficacy than motherwort group（P0.05）.Conclusion： TNF-α is one of the mechanisms that Shenghua Decotion promote cell apoptosis and prevent vaginal bleeding after medical abortion.