血管内皮生长因子与大肠癌淋巴结转移的关系

The correlation of VEGF expression and lymph node metastasis in colorectal carcinoma

目的探讨大肠癌组织中血管内皮生长因子（VEGF）与淋巴结转移的关系。方法采用免疫组化方法检测40份大肠癌组织中VEGF的表达，采用CD34标记计数肿瘤组织微血管密度（MVD），分析。VEGF与大肠癌淋巴结转移的关系，采用χ^2检验和Spearman等级相关分析对相应资料进行分析。结果40例大肠癌患者淋巴结转移24例，MVD为40．65±11．80，VEGF阳性21例（87．5％）；无转移16例，MVD为25．02±11．52，VEGF阳性4例（25．0％），有无淋巴结转移组MVD和VEGF阳性率比较差异均有统计学意义（t=-4．138，χ^2=16．00，P均〈0．01）。40份大肠癌组织中VEGF蛋白表达阳性25例，MYD为41．33±11．61；VEGF阴性15例，MVD为22．84±8．88，组间比较差异有统计学意义（t=5．301，P〈0．05）。大肠癌组织中VEGP的表达与MVD呈正相关（rs=0．539，P〈0．05）。结论。VEGF可能促进大肠癌的血管生成，进而促进大肠癌的转移。

Objective To investigate the correlation between the expression of VEGF and the lymph node metastasis of colorectal carcinoma. Methods VEGF was detected by immunohistochemistry in 24 cases of eolorectal carcinoma with lymph node metastasis and 16 case of coloreetal carcinoma without lymph node metastasis. CD34 was used as a marker to evaluate the MVD. All the data were analyzed using 10. 1 statistical package. The comparison was performed by χ^2 test and .Spearman rank correlation analysis. The level of significance is α = 0.05. Results in the 40 cases of eolorectal carcinoma, 24 cases encountered lymph node metastasis with an MVD （40. 65 ±11.80） and 21 cases were VEGF positive （87.5%）. In the 16 cases without metastasis,the MVD was （25.02 ± 11.52） and 4 cases were VEGF positive （25.0%）. MVD and VEGF were significantly different between the cases with lymph node metastasis and those without （ t = - 4. 138, χ^2 = 16. 00 ,P 〈0. 01 ）. In the 40 cases,there were 25 cases with positive VEGF with an MVD （41.33 ± 11.61 ） and 15 cases with negative VEGF with and MVD （22. 84 ±8.88）. The difference between the cases with positive VEGF and those with negative VEGF （t = 5. 301 ,P 〈 0. 05 ）. VEGF level was positive correlated with MVD in the colon cancer （rs = 0. 539, P 〈 0. 05 ）. Conclusion VEGF may play a role in promoting the lymph node metastasis of coloreetal carcinoma .