SPF种蛋重量对H9亚型禽流感病毒分离培养的影响

Effects of the Weight of Specific Pathogen Free Eggs on the Isolation and Culture of H9 Subtype Avian Influenza Viruses

[目的]探索SPF种蛋重量对病毒分离培养的影响。[方法]以蛋形正常稍重(62～72 g)的SPF种蛋为试验组,适宜重量(50～60g)SPF种蛋为对照组,进行H9亚型禽流感病毒的分离培养试验。[结果]试验组和对照组SPF种蛋的受精率无明显差异,而且尿囊液的HA和HI效价无明显差异。[结论]在实验室可选用稍重的SPF种蛋进行病毒分离培养,既可充分利用SPF种蛋资源,又可节省试验经费。

[Objective] The research aimed to discuss the effects of the weight of SPF eggs on the virus isolation and culture.[Method] Taking SPF eggs with normal egg shape and greater weight（62-72 g） as test group,taking SPF eggs with suitable weight as control group,the isolation and culture test of H9 subtype avian influenza virus was made.[Result] The fertilization rate of SPF eggs in test group and control group had no obvious difference and the titers of HA and HI in allantoic fluid had no obvious difference.[Conclusion] SPF eggs with slightly greater weight could be chosen in the virus isolation and culture,which can make full use of SPF eggs and save the experimental cost.