摘要
[目的]探索SPF种蛋重量对病毒分离培养的影响。[方法]以蛋形正常稍重(62~72 g)的SPF种蛋为试验组,适宜重量(50~60g)SPF种蛋为对照组,进行H9亚型禽流感病毒的分离培养试验。[结果]试验组和对照组SPF种蛋的受精率无明显差异,而且尿囊液的HA和HI效价无明显差异。[结论]在实验室可选用稍重的SPF种蛋进行病毒分离培养,既可充分利用SPF种蛋资源,又可节省试验经费。
[Objective] The research aimed to discuss the effects of the weight of SPF eggs on the virus isolation and culture.[Method] Taking SPF eggs with normal egg shape and greater weight(62-72 g) as test group,taking SPF eggs with suitable weight as control group,the isolation and culture test of H9 subtype avian influenza virus was made.[Result] The fertilization rate of SPF eggs in test group and control group had no obvious difference and the titers of HA and HI in allantoic fluid had no obvious difference.[Conclusion] SPF eggs with slightly greater weight could be chosen in the virus isolation and culture,which can make full use of SPF eggs and save the experimental cost.
出处
《安徽农业科学》
CAS
2012年第1期203-204,共2页
Journal of Anhui Agricultural Sciences