蛹拟青霉发酵菌丝体中虫草酸的提取与测定

Extraction and determination of cordyceps acid in submerged fermentation mycelium of Cordyceps Militaris strains

虫草酸,即D-甘露醇,是虫草类真菌中的主要有效成分之一。为更好开发蛹虫草资源,研究了蛹拟青霉发酵菌丝体中虫草酸的提取工艺。依据大量单因素实验以及正交实验得出最佳提取条件为:微波时间80s,微波功率200W,料液比1:100,乙醇浓度70%。在此条件下,对实验室20株蛹拟青霉进行了虫草酸的测定,结果表明22号菌株虫草酸含量高达160.39mg/g,有一定的生产应用价值。

Cordyceps acid,that is,D-mannitol,is one of the main active ingredients in the type of fungus cordyceps.To explore Cordyceps militaris better,the extracting technology of cordyceps acid in several strains of Cordyceps militaris was determined.According to a large number of single-factor experiments and orthogonal design,the best extraction conditions were drawn：microwave time 80s,microwave power 200W,material-liquid ratio 1：100,ethanol solvent concertration 70%.The strain with the highest acid content among 20 kinds of Cordyceps militaris strains was No.22 strain,whose cordyceps acid content was 160.39mg/g,which had value in production and application.