高速逆流色谱(HSCCC)在丝瓜苷类化合物提取中的应用

Applications of high-speed countercurrent chromatography(HSCCC) in the Luffa cylindrical(L.) Roem glycosides Extraction

丝瓜正丁醇相采用AB-8大孔树脂,利用水和30%、50%、70%和95%的乙醇溶液洗脱提纯得总糖苷。30%洗脱部分以氯仿-甲醇-异丙醇-水(5:6:1:4)为溶剂系统采用高速逆流色谱分离得到4个酯苷类化合物和一个酸,分别为:阿魏酰-β-D-葡萄糖(Ⅰ);1-O-p-香豆酰-β-D-葡萄糖(Ⅱ);对羟基苯甲酰葡萄糖(Ⅲ);咖啡酰-β-D-葡萄糖(Ⅳ);香豆酸(Ⅴ)。50%洗脱部分以氯仿-甲醇-水(13:7:8)为溶剂系统采用高速逆流色谱分离得到2个黄酮苷类化合物,分别为:香叶木素-7-O-β-D-葡萄醛酸苷甲酯(Ⅵ);芹菜素-7-O-β-D-葡萄醛酸苷甲酯(Ⅶ)。70%洗脱部分以氯仿-甲醇-水(13:7:8)为溶剂系统采用高速逆流色谱分离得到1个皂苷化合物丝瓜皂苷H(Ⅷ)。化合物Ⅰ-Ⅶ都是首次从该植物中分离得出。

The n-butyl alcohol phase of Luffa cylindrical（L.） Roem was eluted through AB-8 macroporous adsorption resin with water and 30%,50%,70%,95% ethanol as eluant so as to obtaining the total glucosides compounds.The extraction of 30% ethanol eluant was separated by HSCCC using Vchloroform-Vmethyl alcohol-Visopropyl alcohol-Vwater（5：6：1：4） as the solvent systerm,obtaining 4 ester glucoside compound and one acid as follows：1-Feruloyl-β-D-glucose（Ⅰ）,1-O-p-Coumaroyl-β-D-glucose（Ⅱ）,1-（4-Hydroxybenzoyl）glucose（Ⅲ）,1-Caffeoyl-β-D-glucose（Ⅳ）,p-Coumaric acid（Ⅴ）.The extraction of 50% ethanol eluant was separated by HSCCC using Vchloroform-Vmethyl alcohol-Vwater（13：7：8） as the solvent systerm,obtaining 2 flavonoid glycosides as follows：Diosmetin-7-O-β-D-glucuronide methyl ester（Ⅵ）,Apigenin-7-O-β-D-glucuronide methyl ester（Ⅶ）.The extraction of 70% ethanol eluant was separated by HSCCC using VChloroform-Vmethyl alcohol-Vwater（13：7：8） as the solvent systerm,obtaining Lucyosides H（Ⅷ）.CompoundsⅠ-Ⅷ were isolated for the first time from this plant.