Hansenula anomala转化葡萄糖生产阿拉伯糖醇的研究

Study on production of arabitol from glucose by Hansenula anomala

对Hansenula anomala转化葡萄糖生产D-阿拉伯糖醇的反应条件进行了研究。高供氧量有利于阿拉伯糖醇的生成。在一定范围内,葡萄糖初始浓度越高,阿拉伯糖醇的产量也随之明显提高。并通过正交实验,确定了最佳发酵条件为(g/L):葡萄糖500,酵母膏2,蛋白胨2,CaCl20.5,(NH4)2SO45,MgSO41,KH2PO43,pH5.0;发酵温度30℃,装液量15～20mL/500mL三角瓶,发酵时间263h。阿拉伯糖醇浓度为245.97g/L,转化率为0.49g阿拉伯糖醇/g葡萄糖,平均反应强度0.935g/Lh。

Production of D-arabitol from glucose by Hansenula anomala strain GA24 was studied.Some of the influencing factors were determined：aeration,osmotic pressure and medium.The best fermentation conditions were（g/L）：glucose 500,yeast extract 2,peptone 2,CaCl2 0.5,（NH4）2SO4 5,MgSO4 1,KH2PO4 3,pH5.0;the temperature at 30℃,15～20mL medium contained in 500mL Erlenmeyer flask,and 263h of cultivation.