摘要
利用RACE和克隆等方法得到了虾夷扇贝铜锌超氧化物歧化酶(Cu/Zn-SOD)基因全长cDNA序列,该序列全长1 064 bp,5'和3'非编码区(UTR)分别为61 bp和541 bp,开放阅读框(ORF)462 bp,编码153个氨基酸和一个终止密码子。分析发现,此氨基酸序列含有形成二硫键的两个半胱氨酸残基以及4个铜结合位点、4个锌结合位点,与已知的Cu/Zn-SOD结构相似,与其它物种同源性较高。进行虾夷扇贝组织分布及发育过程中不同时期的实时荧光定量监测,发现鳃中Cu/Zn-SOD基因mRNA相对表达量最高,肾次之,血淋巴中最低;不同发育阶段Cu/Zn-SOD表达量变化明显,其中受精卵和早期D形幼体两个时期表达量相对较高。
A full length cDNA was isolated from Mizuhopecten yessoensis.The cDNA contained an open reading frame（ORF）of 1 064 bp and encoded 153 amino acids.Carried out multiple alignments with other species＇ Cu/Zn-SOD,the predicted amino acid sequence was found to share a high identity with other species.Four copper binding sites and four Zn binding sites were found in amino acid sequence,and the two conservative cysteines which contributed to disulfide bond also showed highly similarities with other species.Real-time PCR was employed to analyze the Cu/Zn-SOD expression in different tissues and during embryonic development stages.As the result showed,the mRNA expression was the highest in gills and lowest in hemocytes.The mRNA expression varied in different growth stages during which the oosperm and early D Larvae were higher.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第12期150-156,共7页
Biotechnology Bulletin
基金
国家海洋局公益性行业专项(200805037)
