海洋红树林内生真菌产抗肿瘤活性化合物1403C发酵条件的优化

Optimization of Fermentation Conditions for Production of Novel Anticancer Compound 1403C in Submerged Culture of Marine Mangrove Endophytic Fungus Halorosellinia sp.

旨在考察pH、溶解氧和剪切力对Halorosellinia sp.(No.1403)发酵过程的影响。结果表明,在发酵中后期及整个发酵过程中控制发酵液的pH恒定对菌体的生长影响不明显,但在不同发酵时期控制不同的恒定pH值对1403C的产量影响较大。仅在发酵的前24 h控制恒pH7.0,有利于菌丝的生长,但抑制1403C的生物合成,菌体干重为对照组的159.1%,1403C的产量仅为对照组的29.4%;从48 h开始控制恒pH7.0,有利于1403C的生物合成,菌体干重量为对照组的94%,1403C的产量为对照组的123.2%;适量的溶解氧和剪切力有利于1403C的生物合成;从48 h开始提高剪切力可获得较高的1403C产量,是对照组的151.8%。综上所述,在发酵至48 h时开始控制恒pH7.0,并适当增大剪切力,在整个发酵过程中控制适当充足的溶解氧有利于1403C的合成。

The effects of different fermentation conditions on the mycelium growth and production of 1403C in submerged culture of Halorosellinia sp.（No.1403）were investigated in this paper.The effects of controlling constant pH during the late phase and the entire fermentation process were not markedly for mycelium growth.However,different strategies of pH control significantly influenced the yield of anticancer compound 1403C.Controlling constant pH7.0 during the first 24 hours was favorable for mycelium growth,but not for biosynthesis of 1403C,the dry cell weight was 159.1% as compared to that of the control,the production of 1403C was only 29.4% as compared to that of the control.Controlling constant pH7.0 from the culture of 48 hours was good for biosynthesis of 1403C and had little influence on mycelium growth,the yield of 1403C was 123.2% as compared to the control and the dry cell weight was 94% as compared to the control.The proper level of DO（dissolved oxygen）and shear stress was of benefit to the biosynthesis of 1403C.Enhancing the shear stress level from the culture of 48 hours could markedly improved the yield of 1403C,which was 151.8% compared to the control.The results above demonstrated that controlling constant pH7.0 and appropriately increasing the shear force from the culture of 48 hours,with enough dissolved oxygen during the whole fermentation process was good for the biosynthesis of 1403C.