摘要
目的探讨8受体在瑞芬太尼诱发大鼠脊髓背角神经元NMDA受体微小兴奋性突触后膜电流（mEPSCs）中的作用。方法取出生14—18d的雄性Wistar大鼠，体重50～60g，取腰段脊髓（L2～S1），制备脊髓切片，取脊髓切片24张，采用随机数字表法，将其随机分为4组（／t=6）：对照组（C组）置入人工脑脊液中培养；甘氨酸组（G组）置人含甘氨酸（终浓度0．24μmol／L）的人工脑脊液中孵育；瑞芬太尼组（R组）制备脊髓切片，置入含瑞芬太尼（终浓度为4nmol／L）的人工脑脊液中孵育；瑞芬太尼＋8受体拮抗剂纳曲吲哚组（RN组）制备脊髓切片，置人含瑞芬太尼和纳曲吲哚（终浓度分别为4nmol／L和1nmol／L）的人工脑脊液中孵育。各组孵育60min后应用全细胞膜片钳技术记录脊髓背角神经元NMDA受体mEPSCs的幅值和频率。结果与C组比较，R组mEPSCs的幅值和频率升高（P〈0．01），G组和RN组差异无统计学意义（P〉0．05）；与R组比较，RN组mEPSCs的幅值和频率降低（P〈0．01）。结论脊髓背角神经元6受体激活后可增强NMDA受体功能，该作用可能参与了瑞芬太尼诱发的痛觉过敏。
Objective To investigate the role of δ-opioid receptor in remifentanil-induced N-methyl-D-as- partate （NMDA） receptor miniature excitatory postsynaptic currents （mEPSCs） in rat spinal dorsal horn neurons. Methods Male 14-18 d old Wistar rats weighing 50-60 g were used in this study. The animals were anesthetized with intraperitoneal choral hydrate 400 mg/kg and sacrificed. Their lumbar segments of spinal cord （LI-S1 ） were immediately removed and sliced. Twenty-four slices were randomly divided into 4 groups （n = 6 each）： control group （group C）; glyeine group （group G）; remifentanil group （group R）＇ and remifentanil ＋ naltrindole（a δ receptor antagon） （group RN）. Slices were cultured in artificial cerebrospinal fluid （ACSF） （group C） or incubated in ACSF containing glycine 0.24 μmol/L （group G） or remifentanil 4 nmol/L （group R） or remifentanil 4 nmol/L ＋ naltrindole 1 nmol/L （group RN） for 60 rain. Whole cell patch clamp technique was used to measure NMDA receptor mEPSCs. Results The amplitude and frequency of mEPSCs were significantly higher in group R than in groups C and RN （ P 〈 0.01 ）. There were no significant differences in amplitude and frequency of mEPSCs among gorups C, G and RN（ P 〉 0.05）. Conclusion Activation of δ-receptor can enhance NMDA receptor function in spinal dorsal horn neurons in rats which may be the mechanism of remifentanil-indueed hyperalgesia.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第11期1344-1346,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（30972847）
天津应用基础及前沿计划研究项目（08JCYBJC08000）
