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环介导逆转录等温扩增技术在输血前HCV检测中的应用 被引量：1

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摘要 目的尝试使用环介导逆转录等温扩增技术用于输血前检测HCV RNA。方法同时采用抗-HCVELISA检测和HCV RT-LAMP检测,比较2者检测结果的差异。结果 2 716例输血前标本,ELISA检测发现19例(0.70%)阳性结果,HCV RT-LAMP检测发现32例(1.18%)阳性结果(P<0.05),2种方法在检测效率上具有显著性差异。RT-LAMP检测在灵敏度上略高于ELISA方法。结论基于HNB颜色反应的环介导逆转录等温扩增技术检测HCV的方法具有快速、灵敏度高的特点。
作者 俞勇 邓丹菲
机构地区 宁波市第二医院输血科
出处 《中国输血杂志》 CAS CSCD 北大核心 2011年第12期1060-1062,共3页 Chinese Journal of Blood Transfusion
关键词 丙型肝炎病毒(HCV) 酶联免疫 环介导逆转录等温扩增
分类号 R446.6 [医药卫生—诊断学] R373.2 [医药卫生—病原生物学]
  • 相关文献

参考文献13

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二级参考文献30

  • 1李启明,马学军,周蕊,彭夫望,高寒春,匡治州,侯云德.环介导逆转录等温扩增技术(RT-LAMP)在丙型肝炎病毒基因检测中的应用[J].病毒学报,2006,22(5):334-338. 被引量：55
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共引文献76

同被引文献12

  • 1李华东,江建宁.实时荧光定量PCR检测HBVcccDNA临床应用研究[J].医学研究杂志,2007,36(12):10-12. 被引量：2
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中国输血杂志
2011年 第12期

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