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苦荞水溶性蛋白体外吸附胆酸盐能力的研究 被引量：24

in vitro Binding of Bile Salts by Water-soluble Proteins from Tartary Buckwheat Grains
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摘要 通过采用硫酸铵盐析法、DEAE-Sepharose Fast Flow离子交换层析法提取分离制备苦荞水溶性蛋白,并通过磷酸盐缓冲液pH值、动态层析流速的最佳选择改善分离提纯效果。同时通过体外吸附胆酸盐能力的测定证实所提取分离得到的苦荞水溶性蛋白具有一定的降血脂功能。结果发现:当磷酸盐缓冲溶液pH值为6.5、流速1.0mL/min于室温下洗脱的效果最好,得到的3个主要分离组分中峰3的体外吸附胆酸盐能力最佳。 Water-soluble proteins were extracted from tartary buckwheat grains by salting out with ammonium sulfate and purified by DEAE-Sepharose Fast Flow chromatography.Optimal conditions for the salting out and DEAE-Sepharose Fast Flow chromatographic separation of water-soluble proteins were determined.The results of experiments on bile salt adsorption in vitro demonstrated that the obtained water-soluble proteins had hypolipidemic effect.Besides,the best results of the elution of water-soluble proteins from DEAE-Sepharose Fast Flow column were achieved using pH 6.5,10 mmol/L phosphate buffer as eluent at a flow rate of 1.0 mL/min and room temperature.Peak 3 was shown to have the best bile salt adsorption ability among three separation peaks obtained.
作者 周小理 黄琳 周一鸣
机构地区 上海应用技术学院香料香精技术与工程学院
出处 《食品科学》 EI CAS CSCD 北大核心 2011年第23期77-81,共5页 Food Science
基金 国家自然科学基金项目(31071527)
关键词 苦荞水溶性蛋白 硫酸铵盐析 DEAE-Sepharose FAST Flow离子交换层析 体外吸附胆酸盐 降血脂 buckwheat water-soluble protein ammonium sulfate salting-out DEAE-Sepharose Fast Flow chromatography in vitro bile salt adsorption lowing blood plasma
分类号 TS201.4 [轻工技术与工程—食品科学]
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参考文献18

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二级参考文献51

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食品科学
2011年 第23期

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