反复冻融增强葛仙米藻胆蛋白抗氧化特性研究

Antioxidant Enhancement of Phycobiliprotein from Nostoc sphaeroides Ku ting by Repeated Freeze-Thawing Treatment

研究反复冻融处理对葛仙米藻胆蛋白抗氧化特性的影响,测定其对抗氧化活性和对H2O2诱导的红细胞溶血和脂质过氧化的抑制作用。结果发现:经过反复冻融处理的葛仙米藻胆蛋白以pH7.3的50mmol/L的磷酸氢二钾-氢氧化钠为溶剂溶解的样品对ABTS+.的抗氧化活性和对H2O2诱导的红细胞溶血和脂质过氧化抑制作用强于其他处理,同时对铁离子的还原能力也较强,主要原因是反复冻融处理增加了葛仙米藻胆蛋白的溶出量,改变其中藻蓝蛋白和藻红蛋白的比例关系。

The present study aimed to investigate the effect of repeated freeze-thawing treatment on the antioxidant capacity of phycobiliprotein from Nostoc sphaeroides Ku ting.Phycobiliproteins extracted from Nostoc sphaeroides Ku ting with and without repeated freeze-thawing treatment were dissolved separately in double distilled water and pH 7.3,50 mol/L dipotassium phosphate-sodium hydroxide buffer,resulting in four different samples： RFTPW,RFTPP,NRFTPW and NRFTPP,respectively.The antioxidant activity of the four samples was comparatively assessed by ABTS＋ radical scavenging assay,FRAP assay and inhibition assay against erythrocyte hemolysis and lipid peroxidation both induced by H2O2.RFTPP was found to have greater inhibitory effects against ABTS＋ radicals,erythrocyte hemolysis and lipid peroxidation than other three samples.Moreover,its ferric reducing activity was stronger.These results may be due to an increase in the dissolved phycobiliprotein caused by repeated freeze-thawing treatment,altering the ratio between phycocyanin and phycoerythrin.