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响应曲面法优化小黄鱼ACE抑制肽的酶解条件 被引量：3

Optimization of Hydrolysis Conditions for Preparation of Small Yellow Croaker-derived Angiotensin-I Converting Enzyme(ACE) Inhibitory Peptides Using Response Surface Methodology
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摘要 以小黄鱼为原料,利用酶解技术制备高活性的ACE抑制肽。通过单因素及响应曲面分析,确定胰蛋白酶(PTN6.0S)的酶解工艺,利用反相高效液相色谱(RP-HPLC)法测定酶解产物(0.05g/L)的ACE抑制率,研究pH值、温度、时间、底物质量浓度、酶与底物比等因素对ACE抑制肽活性的影响。结果显示:最佳工艺条件为pH7.0、温度50℃、时间16.5h、底物质量浓度5.6g/100mL、酶与底物比9‰,酶解产物ACE抑制率达87.36%。 In this study,small yellow croaker meat was hydrolyzed by trypsin（PTN 6.0S） to prepare angiotensin-I converting enzyme（ACE） inhibitory peptides with high activity.Using one-factor-at-a-time method and response surface analysis（RSA）,the optimal hydrolysis conditions for maximum ACE inhibitory rate of hydrolysates were determined as follows： pH 7.0,50 ℃,16.5 h hydrolysis time,5.6 g/100 mL substrate concentration,and 9‰ enzyme/substrate ratio.The ACE inhibitory rate of the hydrolysate obtained under these conditions was determined by RP-HPLC to be 87.36%.
作者 蔡丽丽 徐鑫 刘国艳 何佳易 魏晓蕊 王之颖
机构地区 扬州大学食品科学与工程学院
出处 《食品科学》 EI CAS CSCD 北大核心 2011年第23期167-171,共5页 Food Science
基金 江苏省科技型中小企业技术创新资金项目(BN2008207) 江苏省研究生科研创新计划资助项目(CXLX11-1017)
关键词 小黄鱼 胰蛋白酶 ACE抑制肽 响应曲面法 反相高效液相色谱(RP-HPLC) small yellow croaker trypsin ACE inhibitory peptides response surface methodology RP-HPLC
分类号 TS218 [轻工技术与工程—粮食、油脂及植物蛋白工程]
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参考文献20

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二级参考文献34

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食品科学
2011年 第23期

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