姬菇多糖对CCl_4所致小鼠急性肝损伤的保护作用

Protective Effect of Polysaccharides from Pleurotus cornucopiae on Carbon Tetrachloride-induced Acute Liver Injury in Mice

目的:研究热水浸提法从姬菇中提取姬菇多糖(PCNP)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:小鼠被随机分为空白对照组、模型组、阳性对照组(联苯双酯组,150mg/(kg.d))、处理组(400mg/(kg.d)),连续灌胃30d后,给予CCl4(80mg/kg)建立动物急性肝损伤模型。动物处死后取血液和肝脏测定各项抗氧化指标,以及观察肝组织病理学变化。结果:与模型组相比,处理组能降低血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)活性和肝脏丙二醛(MDA)含量,提高肝脏超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)含量。结论:PCNP能明显改善肝组织病理损伤程度,对四氯化碳(CCl4)所致小鼠急性肝损伤具有明显保护作用。

Objective： To study the protective effect of polysaccharides from Pleurotus cornucopiae（PCNP） on CCl4-induced acute liver injury in mice.Methods： The mice were randomly divided into normal control group and CCl4-induced acute liver injury model group,bifendate treatment group（positive control,at 150 mg/（kg·d）） and PCNP treatment group（400 mg/（kg·d））.The administration of bifendate and PCNP was performed by gavage once a day for 30 days.After the last administration,the model,bifendate treatment and PCNP treatment groups were intragastrically given 1% CCl4 in salad oil solution at the dosage of 80 mg/kg to induce acute liver injury.At 24 h after the administration of CCl4,all the mice were sacrificed to assay antioxidant activities in the blood and liver.Meanwhile,hepatic histopathological changes were also examined.Results： The contents of MDA in the liver and serum AST,ALT and ALP activities revealed an obvious decrease in the PCNP treatment group when compared to the model group,while the contents of GSH and SOD activity in the liver exhibited an obvious increase.Conclusion： PCNP can improve pathological liver injury distinctly and protect against CCl4-induced acute liver injury in mice.