桑白皮总黄酮的抗氧化与镇痛活性研究

Antioxidant and Analgesia Activities of Total Flavonoids from the Root Bark of Morus alba

目的:研究桑白皮总黄酮的抗氧化和镇痛活性。方法:分别采用DPPH自由基法、羟自由基(.OH)法研究桑白皮总黄酮的抗氧化性活性,另外通过小鼠醋酸扭体、热板、甲醛致痛(分别代表3种不同的疼痛模型)研究桑白皮总黄酮的镇痛活性。结果:与VC相比,在体外实验中,桑白皮总黄酮对DPPH自由基和.OH具有良好的清除能力;在体内实验中,桑白皮总黄酮可显著降低由腹腔注射醋酸引起的小鼠扭体次数,同时也可减少热刺激和甲醛诱导的小鼠舔足次数,说明桑白皮总黄酮对化学物质和炎症引起的疼痛均具有一定的缓解作用。结论:桑白皮总黄酮具有显著的抗氧化和镇痛活性。

Objective： To evaluate the antioxidant and analgesic activities of total flavonoids from the root bark of Morus alba.Methods： The antioxidant activity was evaluated by DPPH and hydroxyl free radical scavenging assays using VC as reference standard,and the analgesia activity by acetic acid-induced writhing response,formaldehyde-induced feet-licking response,and heat-induced pain threshold in mice.Results： The total flavonoids had obvious scavenging activity in vitro against DPPH and hydroxyl free radicals.These compounds resulted in a notable decrease in acetic acid-induced writhing number and feet-licking number induced by heat or formaldehyde and consequently had alleviating effect on pain in mice induced by chemicals and inflammatory reaction.Conclusion： Total flavonoids from the root bark of Morus alba have obvious antioxidant and analgesic activities.