山西省娄烦地区北社鼠对杀鼠灵的敏感性研究

Susceptibility of Niviventer confucianus to warfarin in Loufan,Shanxi

目的测定北社鼠对杀鼠灵的敏感性,为检验该鼠的抗药性提供参数。方法实验室做单个笼养无选择摄食试验:雌雄分开,随机分组,按5个不同食毒期供给浓度为0.025%的杀鼠灵毒饵;用Bliss计算机软件,计算不同食毒期与对应死亡率之间的关系。结果北社鼠对杀鼠灵的敏感性,性别之间差异无统计学意义(P_b>0.05,P_(LFP50)>0.05);故将雌雄数据合并计算,得毒力回归方程y=3.29+4.33x,LFP_(50)、LFP_(95)、LF_(99)及其95%置信限分别为2.48(1.97～3.13)、5.96(3.92～9.06)和8.56(4.65～15.78)d。结论按世界卫生组织推荐的以致死99%敏感靶标鼠的食毒期(LF_(90))取整天数作为抗药性检验标准,山西省娄烦地区北社鼠对0.025%的杀鼠灵毒饵摄食期超过9 d存活即为抗性鼠。

Objective To determine the susceptibility of Niviventer confucianus to warfarin. Methods Baits with 0. 025% warfarin were randomly given in single-sex groups of five feeding periods. The time to mortality was analyzed using the Bliss software. Results There was no significant difference in the susceptibility between sexes （Pb〉O.05, PLrPS0〉0.05）. The LFP50, LFP95 and LFP99 of warfarin for N. confucianus were 2.48 （1.97-3.13） days, 5.96 （3.92-9.06） days and 8.56 （4.65-15.78） days, respectively. Conclusion According to the WHO-recommended LFP99-based criteria for rodent warfarin resistance, N. confucianus surviving more than 9 days under exposure to 0.025% warfarin were classified into the resistant strains.