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大鼠皮层小胶质细胞氧糖剥夺模型的制备 被引量:1

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摘要 目的培养Wistar大鼠皮层小胶质细胞,建立氧糖剥夺模型。方法培养Wistar大鼠皮层小胶质细胞,应用OX42抗体进行鉴定,缺氧培养小胶质细胞后测定LDH漏出率。结果小胶质细胞培养至第8天OX42染色阳性细胞为(97.33±2.15)%;氧糖剥夺60min时,LDH漏出率明显增加(P<0.05)。结论成功培养大鼠皮层小胶质细胞,在培养第8天可制备氧糖剥夺模型。
作者 李昕华
出处 《中外医疗》 2011年第35期24-24,共1页 China & Foreign Medical Treatment
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