HPLC法测定山绿茶中芦丁的含量

Content determination of rutin in shanglucha by HPLC

目的:建立山绿茶中芦丁含量的测定方法。方法:采用ODS C18柱,5μm,4.6mm×250mm;以乙腈-体积分数2.5%的醋酸溶液(15∶85)为流动相;流速1.0mL/min;柱温30℃;检测波长254nm。结果:芦丁在0.018 33～0.183 30g/L范围内与峰面积呈良好的线性关系(r=0.999 8,n=5),平均回收率为97.5%(RSD=1.07%,n=6)。结论:该方法简便、准确、重现性好,可用于山绿茶中芦丁含量的测定。

Objective： To establish a method for determination of rutin in shanglucha.Methods： HPLC analysis was carried out using ODS C18 column（4.6 mm×250 mm,5 μm） and acetonitrile-2.5% acetic acid solution（15∶85） as the mobile phase,flow rate 1.0 mL/min,column temperature at 30 ℃.The detection wavelength was 254 nm.Results： Rutin had the good linear relationship with peak area at the range of 0.018 33~0.183 30 g/L（r=0.999 8,n=5）.The average recovery was 97.5%（RSD=1.07%,n=6）.Conclusion： The method is simple,accurate,reproducible and suitable for determining the rutin in shanglucha.