诱导型一氧化氮合酶在宫颈癌组织表达及意义

Expression and clinical significance of iNOS in cervical cancer

目的探讨诱导型一氧化氮合酶iNOS在宫颈癌发生发展中的作用。方法选取宫颈鳞癌组织55例、宫颈上皮内瘤样变及原位癌(CIN)组织31例、正常宫颈组织20例,采用SP免疫组化染色方法检测iNOS的表达。结果宫颈癌组织iNOS表达阳性率明显高于正常宫颈组织、及CIN组织(P<0.05),差异有统计学意义。有淋巴结转移宫颈癌组织的iNOS表达阳性率明显高于无淋巴结转移组织。iNOS表达与宫颈癌的病理分级有关。低分化宫颈癌组织中iNOS表达阳性率高于中高分化癌组织(P<0.05),差异有统计学意义。结论 iNOS表达与宫颈癌的发生发展相关,对宫颈癌的分化及淋巴结转移能起促进作用。

Objective To study the effect of iNOS on the occurrence and development of cervical carcinoma.Methods Immunohistochemical method was used to detect the expressions of iNOS in 55 squamous-cell-cancer（SCC）samples,31 cervical-intraepithelial-neoplasia（CIN）samples and 20 normal-cervical-tissue（NCT）samples.Results The expression rate of iNOS in SCC was manifestly higher than that in the CIN and NCT,the differences were significant（P〈0.05）.The higher expression of iNOS was seen in SCC with lymph-node χ2=4.75,metastasis than that without metastasis（χ2=4.05,P〈0.05）,its expression was associated with the pathological grading of cervical cancer,which was higher in the poorly differentiated than that in the moderately and well-differentiated,the differences were significant（χ2=4.91,P〈0.05）.Conclusions The expression of iNOS is involved in oncogenesis and development of cervical cancer,which may promote the differentiation and lymph node metastasis of this malignancy.