PEG对嗜水气单胞菌疫苗制备与免疫效果影响的研究

Preparation and immune response to the PEG-adjuvanted vaccine against Aeromonas hydrophila in Cryprinuscarpiod

为评价聚乙二醇(PEG)作为超声介质及疫苗佐剂对嗜水气单胞菌疫苗免疫效果的影响,本研究将该疫苗与常规疫苗分别经腹腔注射免疫接种锦鲤成鱼,检测血细胞吞噬百分率、吞噬指数、抗菌活力、抗体效价及免疫保护力等指标,评价疫苗效果。结果表明PEG6000作为佐剂制备的疫苗,其各指标均高于对照组及其他试验组。PEG6000疫苗免疫保护力达到77.8%,高于常规灭活疫苗22.2%,表明PEG6000作为佐剂制备的疫苗效果最好。

Aeromonas hydrophila was recognized as a human pathogen for decades.However,it still remains under development for an effective vaccine against this bacterium.The aim of this study was to investigate the impact of PEG as an ultrasonic carrier and vaccine adjuvant on the immune protection of vaccine against A.hydrophila.Cryprinuscarpiod was injected intraperitioneally with control vaccine and PEG-conjugated vaccine,respectively.The percentage and index of phagocytosis,antibacterial activities,antibody titer and the relative survival percentage were thereafter examined.The results showed that all the immune response indicators of PEG6000-conjugated vaccine were significantly higher than those of control vaccine.Particularly,the relative survival percentage of PEG6000-conjugated vaccine reached 77.8%,which was 22.2% higher than that of inactivated control vaccine.Our results indicate that PEG6000 is a highly effective ultrasonic carrier and adjuvant in the preparation of vaccine against A.hydrophila.