人胎盘源间充质干细胞对脐血CD8^+T细胞活化及周期和IL-17分泌的影响

The effect of human placenta-derived mesenchymal stem cells on cord blood CD8^+T cells activation,cellcycle and secretion of IL-17

目的:探讨人胎盘源间充质干细胞(hPMSCs)对脐血CD8+T细胞活化、周期及IL-17分泌的调节作用,为其在临床细胞治疗中的应用提供理论依据。方法:应用消化法分离、培养hPMSCs,体外扩增培养3代后用于实验;应用免疫磁珠法分选脐血CD8+T细胞;应用流式细胞术(FCM)分析hPMSCs对植物血凝素(PHA)刺激下脐血CD8+T细胞早期表型CD25、CD69表达和细胞周期的影响;佛波酯(PMA)刺激下脐血CD8+T细胞对IL-17分泌的影响。结果:hPMSCs体外可使脐血CD8+T细胞滞留于细胞周期的G0/G1期,下调活化脐血CD8+T细胞早期表型CD25、CD69的表达,上调其IL-17的分泌。结论:hPMSCs体外可通过对脐血CD8+T细胞周期的影响抑制其活化,并且上调脐血CD8+T细胞IL-17的分泌。

AIM： To study the effect of human placenta- derived mesenchymal stem cells （hPMSCs） on cord blood CD8^＋ T cell activation, cell cycle and secretion of IL-17, and to provide the theoretical basis for it application in the cell- based therapies. METHODS： hPMSCs were isolated from mature placenta by the method of digestion. Then hPMSCs were cultured, expanded in vitro, and were used in test after the third passage. CD8 ^＋T cells were sorted from cord blood with immunomagetic beads. FCM was used to analyze the expression of early activation phenotype, cell cycle of cord blood CD8^＋ T cells and cytokine secretion. RESULTS： CD8^＋T cells stimulated by PHA in the presence of hPMSCs were arrested at G0/G1 phase. The expression of the early activation marker CD25 and CD69 of cord blood CD8^＋T cells was inhibited in the presence of hPMSCs. While, IL-17secretion of cord blood CD8^＋T cells stimulated by PMA was increased. CONCLUSION： hPMSCs can suppress the activation of cord blood CD8^＋T cells by altering T cell cycle; up-regulate the level of IL-17 secreted by cord blood CD8^＋ T cells.