肾透明细胞癌中AnnexinⅡ的表达及其临床意义

The expression and clinical significance of Annexin Ⅱ in clear-cell renal cell carcinoma

目的:探讨AnnexinⅡ在人肾透明细胞癌和癌旁组织样本中的表达情况及其临床意义。方法:采用Western blot方法检测29例新鲜肾透明细胞癌及相应癌旁组织中AnnexinⅡ蛋白的表达;采用免疫组织化学方法检测120例肾透明细胞癌及相应癌旁组织中AnnexinⅡ的表达情况,并将结果与患者的临床病理参数及预后进行相关性分析。结果:AnnexinⅡ在肾透明细胞癌及相应癌旁组织中均有表达,前者的表达水平较后者显著升高(P<0.01);免疫组织化学显示肾透明细胞癌组织中,AnnexinⅡ的阳性表达率高达67.5%,与癌旁组织存在明显差异(P<0.01);AnnexinⅡ在肾透明细胞癌组织中以胞膜表达为主,其表达状况与肾透明细胞癌患者的临床分期(P<0.05),组织学分级(P<0.05),肾被膜受侵(P<0.01)和远地转移(P<0.01)紧密相关;并且AnnexinⅡ的表达状况与肾透明细胞癌患者的5年生存率显著相关。结论:AnnexinⅡ在肾透明细胞癌组织中过表达,其与肾癌的发展密切相关,在肾癌的侵袭及转移过程中可能发挥重要作用。

AIM： To discuss the difference of annexin II expression between the Clear-Cell Renal Cell Carcinoma （ccRCC） and the corresponding normal renal tissues, and to reveal the clinical significance of Annexin II expression in ccRCC. METHODS： Western blotting was used to detect the expression of Annexin II in 29 samples of fresh ccRCC and the corresponding normal renal tissues. Annexin II expression was examined in 120 samples of paraffin-embed- ding ccRCC and the corresponding normal renal tissues using immunohistochemistry. Correlations with Annexin II expression and clinic-pathological parameters were analyzed. RESULTS： There was expression of Annexin II in both ccRCC and the corresponding normal renal tissues. In ccRCC tissues, the total levels of Annexin II proteins were significantly higher than that in the corresponding normal renal tissues （ P 〈 0.01）. The positive rate of Annexin II expression in immunohistochemistry was 67.5% in the ccRCC tissues, existing significant difference compared with the corresponding normal renal tissues （ P 〈 0.01 ）. Expression of Annexin II in ccRCC mainly showed membranous staining. Annexin II expression level was positively correlated with TNM stage （ P 〈 0.05 ）, histology grade （ P 〈 0.05）, renal capsule infiltration （ P 〈 0.0] ） and distant metastasis （P〈 0.01） of ccRCC. Moreover, Annexin II expression level was significantly correlated with ccRCC patients＇ 5- years survival rate （ P 〈 0.01 ）. CONCLUSION. Overexpression of Annexin II in the ccRCC is closely correlated with the ccRCC development. It may play an important role in the process of ccRCC invasion and metastasis.