子宫内膜癌中microRNA-125b对HER2基因3'非翻译区的调控作用

The role of microRNA-125b on HER2 gene 3'-untranslated region in endometrial cancer

目的:明确miR-125b在子宫内膜癌中对HER2基因3'UTR的靶向调控作用。方法:分别构建野生型和突变型HER2基因3'UTR荧光素酶载体;软件预测HER2基因3'UTR靶向miRNAs;共转染miR-125b和荧光素酶载体于HEC-1B细胞中,双荧光素酶检测系统测定荧光素酶活性。结果:通过软件预测在HER2 3'UTR第37bp位置有一个miR-125b结合位点;与转染对照miR组和突变型载体组相比,miR-125b前体能明显降低野生型载体的荧光素酶活性。结论:miR-125b能够靶向负性调控HER2基因3'UTR活性。

Objective：To identify the regulation of miR-125b to HER2 gene 3＇UTR in endometrial cancer.Methods： The wild type and mutation type of HER2 gene 3＇UTR luciferase vectors were constructed.Software was applied to predict the HER2 3＇UTR targeting miRNAs.The dual luciferase assay system was used to detect the reporter activity followed by co-transfecting the luciferase vector constructed and pre-miRNA into HEC-1B cells.Results： MiRNA targeting prediction showed that the 37th bp in 3＇UTR contained a miR-125b binding site.The luciferase assay revealed that pre-miR-125b could significantly decrease the luciferase activity of wild type HER2 3＇UTR vector,compared with the control and mutation vector transfection groups,P0.05.Conclusion： MiR-125b could negatively target and regulate the activity of HER2 gene 3＇UTR.