三个牦牛群体DRB3.2基因PCR-RFLP多态性研究

The polymorphism study of DRB3.2 gene by PCR-RFLP in three yak populations

为了研究牦牛DRB3.2基因exon 2遗传多样性,试验采用PCR-RFLP对天祝白牦牛、甘南牦牛、大通牦牛3个类群757头个体的MHC-DRB3.2基因进行PCR-RFLP分析。结果表明:共检测出8个HaeⅢ酶切位点、11种基因型;在3个牦牛类群中,HaeⅢC基因型(225 bp/175 bp/85 bp/35 bp)在大通牦牛、天祝白牦牛中是优势基因型,基因型频率分别为0.387和0.366;而在甘南牦牛中,HaeⅢA基因型(175 bp/85 bp/35 bp)为优势基因型,基因型频率为0.306。3个群体的多态信息含量分别为0.739,0.754,0.743,均达到了高度多态(PIC>0.50)。说明牦牛DRB3.2基因具有高度多态性,在研究牦牛抗病育种和提高牦牛生产性能方面具有独特的效力和广泛的应用前景。

To study the genetic diversity of DRB3.2 gene, PCR - RFLP was used to analyze the MHC - DRB3.2 genes from 757 yak individuals belonged to three different groups （Tianzhu white yak, Gannan yak and Datong yak）. The results showed that 8 Hae Ⅲ restriction site and 11 genotype were detected, Hae Ⅲ C （225 bp/175 bp/85 bp/35 bp） was the dominant genotype in Datong yak and Tianzhu white yak,its frequencies were 0. 387 and 0. 366 respectively. Hae m A （175 bp/85 bp/35 bp） was the dominant genotype in Gannan Yak,its frequency was 0. 306. The Polymorphic information contents of three different groups were 0. 739,0. 754 and 0. 743 ,respectively. All of them reached high poly- morphism （ PIC 〉 0.50）. The results indicates that DRB3.2 gene is highly polymorphic, it has a unique effect and wide application prospects of breeding for disease resistance and raising the performance of yak.