摘要
为了研究H9N2亚型AIV A/Chicken/Guangdong/333/2008的全基因组序列变异情况,试验利用RT-PCR方法扩增出该病毒的8个基因序列,并应用DNAStar和MEGA4.0软件分析所得基因序列。结果表明:该病毒HA基因的第226位氨基酸由Q(Gln)变为了L(Leu),其NP基因与Viet Nam/1203/2004(H5N1)的NP基因同源率最高。说明该病毒已具备了感染哺乳动物的分子特征,并可能在遗传演化过程中突变为高致病性的禽流感病毒(AIV)。
To study the whole genome sequence variation of H9N2 subtype AIV A/Chicken/Guangdong/333/2008, the eight gene sequences from a H9N2 subtype AIV were amplified by RT - PCR,and analyzed by DNAStar and MEGA 4.0. The results showed that the amino acid at position 226 of HA turned Q to L,and the sequence of NP was most similar with the NP of Viet Nam/1203/2004( H5N1 ) ,which isolated from human. The results revealed that the virus had the molecular characteristics to infect mammals,it might mutate into highly pathogenic avian in- fluenza(AIV) in the process of genetic evolution.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2012年第1期14-16,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
国家肉鸡产业技术体系项目(nycytx-42-G3-03)
广东省科技计划项目(2009B020307002
2009A0201006)