一株H9N2亚型禽流感病毒全基因组分析

Analysis of full-length genes of a H9N2 subtype Avian influenza virus

为了研究H9N2亚型AIV A/Chicken/Guangdong/333/2008的全基因组序列变异情况,试验利用RT-PCR方法扩增出该病毒的8个基因序列,并应用DNAStar和MEGA4.0软件分析所得基因序列。结果表明:该病毒HA基因的第226位氨基酸由Q(Gln)变为了L(Leu),其NP基因与Viet Nam/1203/2004(H5N1)的NP基因同源率最高。说明该病毒已具备了感染哺乳动物的分子特征,并可能在遗传演化过程中突变为高致病性的禽流感病毒(AIV)。

To study the whole genome sequence variation of H9N2 subtype AIV A/Chicken/Guangdong/333/2008, the eight gene sequences from a H9N2 subtype AIV were amplified by RT - PCR,and analyzed by DNAStar and MEGA 4.0. The results showed that the amino acid at position 226 of HA turned Q to L,and the sequence of NP was most similar with the NP of Viet Nam/1203/2004（ H5N1 ） ,which isolated from human. The results revealed that the virus had the molecular characteristics to infect mammals,it might mutate into highly pathogenic avian in- fluenza（AIV） in the process of genetic evolution.