灰额刺尾鱼溃疡病病原分离及鉴定

Identification of the pathogen isolated from the skin-ulcered Acanthurus glaucopareius

从灰额刺尾鱼(Acanthurus glaucopareius)体表溃烂处及内脏器官中分离到5株较强致病力的菌株,经过人工感染健康鱼证实该菌株是致病菌。对该病原菌进行了形态、生理生化反应测试,结果显示为溶藻弧菌(Vibrioalginolyticus)。应用细菌16S rDNA的通用引物对该菌基因组DNA进行PCR扩增、克隆测序,得到1条长度为1 392 bp的核苷酸序列,该序列与溶藻弧菌(登录号GQ455008)的16S rDNA基因序列同源性达99%,进一步确定该致病菌为溶藻弧菌。药敏试验结果表明,该菌株对痢特灵、氟哌酸、环丙沙星等药物敏感,对甲氧胺嘧啶、氨苄青霉素、阿米卡星、青霉素等药物耐药。研究亮点:通过系统的病原学试验研究,证实了灰额刺尾鱼溃疡病的病原为溶藻弧菌,并验证了该病原菌的药物敏感性等特性,以及对该病的病症、病因及临床治疗方法进行了分析,这些结论尚属首次报道,为灰额刺尾鱼溃疡病以及其他海水观赏鱼同类疾病的临床诊断和防治技术提供科学依据。

Five bacteria strains were isolated from the skin ulcer and internal organ of the Acanthurus glaucopareius.The artificial infection test proved that the isolated strain was the pathogenic bacterium.Its morphological,physiological and biochemical characteristics were studied,and they completely matched Vibrio alginolyticus.The 16S rDNA gene was amplified by using the universal primers,and the PCR product was cloned and sequenced.A sequence of 1 392 base pair（bp） was obtained from the bacteria.The sequence was similar to the sequence of Vibrio alginolyticus（accession No.GQ455008）,and the similarity was 99%.Vibrio alginolyticus was confirmed to be the pathogenic bacterium.The drug sensitivity test of bacteria strain revealed that the pathogen was sensitive to drugs such as furazolidone,norfloxacin and ciprofloxacin,and the pathogen was resistant to drugs such as methoxamine pyrimidine,ampicillin,amikacin and penicillin.