FAT对K562和K562/A02细胞内药物积聚浓度的影响

Effect of FAT on Intracellular Accumulation of Drug in K562/A02 Cells

目的:寻找克服肿瘤细胞多药耐药的方法。方法:以FAT作为耐药逆转剂,采用流式细胞术,观察FAT对K562和K562/A02内药物的积聚和外排的影响。结果:FAT可增加K562/A02细胞内罗丹明123(Rhodamine 123)的含量,且存在剂量依赖关系,而对于K562细胞无明显影响。结论:FAT有望成为一种新型多药耐药逆转剂。

Objective：To explore measures to overcome multidrug resistance of tumor ceils. Methods：The effect of FAT on intracellular drug accumulation and drug efflux in K562 cell line and K562/A02 cell line was studied by flow cytometry. Results：FAT increased the intracellular Rho-123 concentration,the role of drug resistance reversal was FAT dose-dependant. The content of Rho- 123 was not influenced by FAT in K562 cell line nearly. Conclusion：FAT is a novel multidrug resistance reversal agents.