蛇床组织培养及无性系的建立

Study on Tissue Culture and Establishment of Clone for Cnidium monnieri

为保护野生资源,实现人工栽培,以蛇床嫩茎的鳞茎为材料,采用组织培养的方法,进行了愈伤组织诱导、不定芽分化、试管苗生根、移栽和定植的研究,建立起了蛇床无性系。结果表明,MS+6-BA0.2 mg/L+2,4-D 1.5 mg/L+NAA 0.5 mg/L是嫩茎愈伤组织诱导培养和继代培养的理想培养基;1/2 MS+AgNO31.2 mg/L+6-BA 0.8 mg/L+NAA 0.1 mg/L+GA30.5 mg/L是愈伤组织分化培养和不定芽分化继代增殖培养的理想培养基;White+IBA 0.1 mg/L+IAA 0.2 mg/L是不定芽生根培养的理想培养基。其试管苗的繁殖速度可达每年50万株,定植的试管苗保持了野生蛇床的所有植物学性状。

To protect wild resources and realize artificial cultivation, by using tissue culture methods, the tender stems of Cnidiurn monnieri were used as materials to do the research on callus induction, adventitious buds differentiation, rooting and transplanting of tube seedlings and finally established the clone of Cnidium monnieri. The results showed that MS ＋ 6-BA 0.2 mg/L ＋ 2,4-D 1.5 mg/L ＋ NAA 0.5 mg/L, 1/2 MS ＋ AgNO3 1.2 mg/L ＋ 6-BA 0.8 mg/L ＋ NAA 0.1 mg/L ＋ GA3 0.5 mg/L and White ＋ IBA 0.1 mg/L ＋ IAA 0.2 mg/L were the optimum media for callus induction, adventitious buds differentiation and rooting respectively. The propagation speed of tube seedlings could be 500 000 plants per year. Colonization of plantlets maintained all biological traits which wild plant has.