人血浆中孟鲁司特的LC-MS/MS法测定

Determination of Montelukast in Human Plasma by LC-MS/MS

建立了液相色谱-串联质谱法测定人血浆中的孟鲁司特。采用C18色谱柱,以辛伐他汀为内标,乙腈-水-甲酸(86︰14︰0.3)为流动相,ESI源正离子检测方式,多反应监测,监测离子对m/z 586.3→422.5(孟鲁司特)、m/z441.4→325.2(辛伐他汀)。孟鲁司特在1～1 000 ng/ml浓度范围内线性关系良好。回收率为96.7%～110.6%,RSD小于7.0%。

A LC-MS/MS method was established for the determination of montelukast in human plasma. A C18 column was used with the mobile phase of acetonitrile-water-formic acid （86 ： 14 ： 0.3） and simvastatin as the internal standard. Multiple reaction monitoring （MRM） mode was used with the transitions of m/z 586.3→422.5 （montelukast） and m/z 441.4→325.2 （simvastatin）. The calibration curve was linear in the concentration range of 1 - 1 000 ng/ml. The recoveries were 96.7 % - 110.6 %, with RSD less than 7.0 %.