长寿命浆细胞与特发性血小板减少性紫癜患者预后的相关研究

Relevant research of long - lived plasma cells and the prognosis of patients with idiopathic thrombocytopenic purpura

目的探讨长寿命浆细胞与特发性血小板减少性紫癜（ITP）患者预后，及其与相关临床指标的关系。方法用流式细胞术检测100例初诊ITP患者和20例正常对照者外周血长寿命浆细胞表达率。随访6个月后，将ITP患者分为急性型与慢性型两组，比较初诊时外周血长寿命浆细胞表达率，并分析长寿命浆细胞表达率与就诊时间、初诊时血小板计数、血小板相关抗体、骨髓巨核细胞计数、治疗反应的关系。结果外周血长寿命浆细胞表达率，急性型组与对照组比较差异无统计学意义（P〉0．05），慢性型组明显高于对照组，差异有统计学意义（P〈0．05）；慢性型患者外周血长寿命浆细胞表达率与初诊时血小板计数、血小板相关抗体无相关（P〉0．05），与就诊时间、骨髓巨核细胞计数、治疗反应相关（P〈0．05）。结论长寿命浆细胞在慢性型ITP患者外周血中高表达，可作为早期判断ITP患者预后的重要指标之一。

Objective To investigate the relationship of the long - lived plasma cells and the prognosis of patients with idiopathic thrombocytopenic purpura （ITP） , and the relationship with ITP＇ s relevant clinical indicators. Methods The expression rate of long -lived plasma cell in peripheral blood of 100 patients with preliminary diagnosis of ITP and 20 normal controls were assayed by flow cytometry. After follow - up of six months, the ITP patients were divided into acute - type and chronic - type two groups, the expression rate of long - lived plasma cells in peripheral blood when preliminary diagnosed were compared, and the relationships between expression rate and treatment time, platelet count when preliminary diagnosis, platelet- associated antibody, bone marrow megakaryocyte count, therapeutic reaction were analyzed. Results Expression rate of long - lived plasma cells in peripheral blood had no significant difference （P 〉 0.05 ） in acute -type group and the control group, and it was significantly higher in chronic -type group than that in control type, the difference was statistically significant （P 〈 0.05 ） ; Expression rate of long - lived plasma cells in peripheral blood in chronic - type group had no correlation with platelet count when preliminary diagnosis and platelet- associated antibodies （P 〉 0.05 ）, and was correlated with the treatment time, bone marrow megakaryocyte count, therapeutic reaction （P 〈 0.05 ）. Conclusions Long - lived plasma cells in peripheral blood of patients with chronic ITP patients has high expression, which can be used as one of the important indicators for early determination of the prognosis of patients with ITP.