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乳头状甲状腺癌中谷胱甘肽过氧化物酶3启动子区高甲基化与表达缺失 被引量:12

Glutathione peroxidase 3 promoter region hypermethylation and loss of expression in papillary thy-roid carcinomas
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摘要 目的探讨乳头状甲状腺癌(PTC)中谷胱甘肽过氧化物酶3(GPX3)基因启动子区域甲摹化状态,分析GPX3甲基化与其基因表达的关系。方法应用甲基化特异性聚合酶链式反应(MSP)检测47例PTC、10例非癌组织标本和乳头状甲状腺癌细胞株TPC-1中GPX3的甲基化状态,并用逆转录-聚合酶链式反应(RT—PCR)检测TPC-1细胞中GPX3的mRNA表达。结果48.9%(23/47)PTC发生GPX3基因启动子区域甲基化,而10例非癌组织均未发生甲基化;TPC-1中GPX3启动子区甲基化,而且GPX3的mRNA不表达,经5-aza-2’-deoxycytidine干预96h后GPX3重新表达。结论GPX3基因启动子区域频繁发生甲基化,可能作为PTC的诊断标志物;GPX3启动子区的甲基化是其基因表达的重要调节机制。 Objective To explore the station of glutathione peroxidase 3 (GPX3) promoter region methylation in papillary thyroid carcinoma (PTC) and analyze the relationship between the expression of GPX3 and its methylation. Methods Methylation-specifie polymerase chain reaction (MSP) was employed to detect GPX3 gene promoter region methylation in 47 papillary thyroid carcinoma samples, 10 non-cancerous tissues and the papillary thyroid carcinoma cell line TPC-1. By using reverse transcription polymerase chain reaction (RT-PCR) the mRNA expression of GPX3 wad detected in TPC-1 cells. Results 48. 9% (23/47) of PTC was methylated, while none of the 10 non-cancerous tissues was methylated. The promoter of GPX3 was hypermethylated in TPC-1 cells, and after treatment of TPC-1 with 5-aza-2' -deoxycytidine for 96 h, the mRNA of GPX3 was re-expressed. Conclusion GPX3 promoter region methylation probably become PTC diagnostic marker. GPX3 promoter hypermethylation is an important epigenetic regulation meehanism of its mRNA expression.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2012年第1期77-79,共3页 Chinese Journal of Experimental Surgery
关键词 DNA甲基化 谷胱甘肽过氧化物酶3 甲状腺癌 DNA methylation Glutathione peroxidase 3
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  • 1Woodruff SL,Arowolo OA,Akute OO,et al. Global variation in the pattern of differentiated thyroid cancer. Am J Surg,2010 ,200 :462-466.
  • 2Davies L, Welch HG. Increasing incidence of thyroid cancer in the United States, 1973-2002. JAMA ,2006,295:2164-2167.
  • 3Wang JY, Yang IP, Wu DC, et al. Functional glutathione peroxidase 3 polymorphisms associated with increased risk of Taiwan Residents patients with gastric cancer. Clin Chim Acta,2010,411 : 1432-1436.
  • 4Brigelius-Flohe R, Kipp A. Glutathione peroxidases in different stages of carcinogenesis. Biochim Biophys Acta,2009,1790 : 1555-1568.
  • 5Jones PA, Laird PW. Cancer epigenetics comes of age. Nat Genet, 1999,21:163-167.
  • 6Paz MF, Fraga MF, Avila S, et al. A systematic profile of DNA methyl- ation in human cancer cell lines. Cancer Res,2003,63 : 1114-1121.
  • 7Fleisher AS, Esteller M, Tamura G, et al. Hypermethylation of the bMLH1 gene promoter is associated with microsatellite instability in early human gastric neoplasia. Oncogene ,2001,20:329-335.
  • 8陈新军,袁先厚,吴勉云,江普查,马超,文志华.人脑胶质瘤U251细胞中hMLH1、hMSH2基因启动子区的甲基化状态研究[J].中华实验外科杂志,2007,24(11):1300-1302. 被引量:4
  • 9陈新军,袁先厚,江普查,张捷,马超,文志华.脑胶质瘤中hMLH1、hMSH2基因启动子区的甲基化状态研究[J].中华实验外科杂志,2010,27(4):478-480. 被引量:1
  • 10Catteau A, Morris JR. BRCA1 methylation:A significant role in tumour development? Semin Cancer Bio1,2002 ,12 :359-371.

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  • 1卢秀波,安兆峰,王庆兆,殷德涛,邱新光.Survivin和c-myc在分化型甲状腺癌中的表达及临床意义[J].中华实验外科杂志,2004,21(6):705-706. 被引量:18
  • 2李常海,陈孝平,黄志勇,何松青.肝移植治疗原发性肝癌的现状[J].中华实验外科杂志,2005,22(5):636-638. 被引量:16
  • 3刘涛,杨维良,张新晨,张东伟.肝癌缺失基因在胃癌中的表达及启动子甲基化[J].中华实验外科杂志,2005,22(7):822-824. 被引量:11
  • 4胡礼炳,王国民,张利能,查锡良.膀胱癌细胞中DAPK基因启动子甲基化状态分析[J].中华实验外科杂志,2007,24(4):489-491. 被引量:13
  • 5Yin DT,Wang L, Sun J, et al. Association of the promoter methylation and protein expression of Fragile Histidine Triad (FHIT) gene with the progression of differentiated thyroid carcinoma. Int J Clin Exp Patho1,2010,3:482-491.
  • 6Stephen JK, Chitale D, Narra V, et al. DNA methylation in thyroid tu- morigenesis. Cancers(Basel) ,2011,3 : 1732-1743.
  • 7Lal G, Padmanabha L, Smith B J, et al. RIZ1 is epigenetically inactiva- ted by promoter. Cancer,2006,107:2752-2759.
  • 8De Brouwer S, Mestdagh P, Lambertz I,et al. Dickkopf-3 is regulated by the MYCN-induced miR-17-92 cluster in neurohlastoma, lnt J Cancer,2012,130:2591-2598.
  • 9Veeck J,Bektas N, Hartmann A,et al. Wnt signaling in human breast cancer:expression of the putative Wnt inhibitor Dickkopf(DKK3) is frequently suppressed by promoter hypermrthylation in mammary tumors. Breast Cancer Res,2008,10 : R82.
  • 10Yin DT,Wang L, Sun J, et al. Homozygous deletion but not mutation of exons 5 and 8 of the fragile histidine triad (FHIT) gene is associat- ed with features of differentiated thyroid carcinoma. Ann Clin Lab Sci ,2010,40:267-272.

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