巨噬细胞移动抑制因子过表达对成骨细胞骨形态蛋白-2和血管内皮生长因子表达的影响

Effects of overexpression of macrophage migration inhibitory factor on bone morphogenetic protein- 2 and vascular endothelial growth factor in osteoblasts

目的观察巨噬细胞移动抑制因了（MIF）在成骨细胞中的表达及其对成骨细胞骨形态蛋白-2（BMP-2）和血管内皮生长因子（VECF）表达的影响。方法提取5例人髂骨成骨细胞，PcDNA3．0构建MIF过表达系统导入成骨细胞中，检测MIF、BMP-2和VEGF的表达。结果MIF的稳定转染使成骨细胞的MIF过表达（P〈0．05）。MIF基因mRNA和蛋白表达在转染组的表达量显著高于对照组中的表达量（P〈0．05）；VEGF和BMP-2的mRNA和蛋白的表达量在转染组也明显增高（P〈0．05），结论MIF过表达在成骨细胞中上谳VEGF和BMP-2的表达。

Objective To discuss the overexpression of macrophage migration inhibitory factor （MIF） on bone morphogenetic protein-2 （BMP-2） and vascular endothelial growtb factor, tor（VECF） in oste-oblasts. Methods Human osteoblasts were isolated from 5 patients, and then pcDNA3.0-MIF was constructed and transfected into osteoblasts. The effect of MIF overexpression on BMP-2 and VEGF in osteo-blasts was detected. Results MIF overexpression was verified （P 〈0. 05）. MIF, and BMP-2 and VEGF mRNA and protein levels were higher in transfected cells than in control cells （P 〈 0. 05）. Conclusion Overexpression of MIF up-regulated the BMP-2 and VEGF in osteoblasts.