中药蒲公英基因组总DNA提取方法研究

Extraction of genomic DNA from Taraxacum Officinale

目的寻找一种快速简便的蒲公英干品总DNA的提取方法。方法用改良CTAB、SDS、高盐低pH 3种提取方法及液氮、玻璃砂2种研磨方法提取蒲公英总DNA,采用蛋白核酸测定及电泳检测所得DNA的纯度和浓度。结果 3种提取方法所得到的DNA纯度和浓度不同,改良CTAB法提取总DNA纯度和浓度较高,电泳显示主条带较清晰,降解较少;2种研磨方法蛋白核酸测定及电泳结果相近。结论改良CTAB法适用于蒲公英药材基因组DNA的提取。

Objective To develop a quick and convenient method for the extraction of total DNA of Taraxacum Officinale.Methods Three isolation methods including modified CTAB method,SDS method and low pH medium with high salt method and the two grinding methods of nitrogen and glass-sand were used for DNA extraction.The purity and concentration of DNA were analyzed with UV absorption and electrophoresis method.Results The DNA purity and concentration were different.The result of modified CTAB method was the best.The strips were clear and less decomposed in agarose electrophoresis.The results of the two grinding methods were similar.Conclusion The modified CTAB method is suitable for the extraction of genomic DNA from Taraxacum Officinale.