肝细胞癌中多药耐药基因1表达与乙型肝炎病毒感染关系的研究

Relationship between the expression of multidrug resistance gene 1 in hepatocellular cancer and hepatitis B virus infection

目的探讨肝细胞癌中多药耐药基因1(multidrug resistance gene 1,MDR1)的表达特点及其与乙型肝炎病毒(hepatitis B virus,HBV)感染的关系。方法 51例肝癌患者癌组织标本(肝癌组)和10份正常肝组织标本(对照组),运用荧光定量PCR技术检测2组MDR1基因的表达情况,分析MDR1基因表达与HBV感染间的关系。结果肝癌组MDR1基因表达(0.55±0.27)高于对照组(0.23±0.10),差异有统计学意义(P<0.05);MDR1表达随肿瘤Edmondson分级增高而增高(P<0.05);肝癌组HBsAg阳性患者MDR1表达高于HBsAg阴性患者(P<0.05)。结论MDR1高表达在肝细胞癌原发性耐药中可能起重要作用,HBV可作为肝细胞癌化疗耐药的参考指标之一。

Objective To detect the expression of multidrug resistance gene 1（MDR1） gene and hepatitis B virus（HBV） infection in hepatocellular cancer and evaluate the relationship between them.Methods Fluorescent quantitation PCR was used to examine the expression of MDR1 in 51 cases of hepatocellular carcinoma and 10 cases of normal tissue.The relationship between the expression of MDR1 and HBV infection was analyzed.Results The expression of MDR1 mRNA was higher in hepatocellular carcinoma patients（0.55±0.27） than that in normal tissue（0.23±0.10）,which showed a significant difference（P0.05）.And the expression of MDR1 gene was positively correlated with tumor Edmondson grading（P0.05）.The expression of MDR1 was significantly higher in positive HBV patients than that in negative HBV patients（P0.05）.Conclusion The higher expression of MDR1 may play an important role in the primary multidrug resistance of hepatocellular carcinoma.HBV can be used as a useful index in chemotherapy resistance.