注射用姜黄素温敏凝胶在实体瘤内滞留时间的研究

Retention Time of Curcumin Thermosensitive Hydrogels for Injection in Solid Tumor

目的:研究注射用姜黄素温敏凝胶在小鼠实体瘤内的滞留时间。方法:复制小鼠实体瘤模型,以亚甲蓝温敏凝胶和亚甲蓝水溶液为模型药物,直观考察温敏凝胶是否可延长滞留时间,确定所用方法的可行性。通过反相高效液相色谱法测定注射用姜黄素温敏凝胶于不同时间点时在小鼠实体瘤内的剩余量,从而确定其滞留时间,并与同浓度的注射用姜黄素混悬液对比。结果:亚甲蓝水溶液注射进入瘤体后会沿着针孔外渗,而亚甲蓝温敏凝胶无此现象。注射用姜黄素温敏凝胶在实体瘤内的滞留时间为43.02h,显著长于姜黄素混悬液的滞留时间(P<0.01)。结论:注射用姜黄素温敏凝胶在小鼠实体瘤内滞留时间较长,显示其具有一定的缓释作用。

OBJECTIVE： To investigate the retention time of Curcumin thermosensitive hydrogels for injection （CTH） in solid tumor. METHODS： Solid tumor model was induced. Methylenum coeruleum thermosensitive hydrogels （MCTH） and solutions （MCS） were prepared as model drug to discuss whether the thermosensitive hydrogels could enhance the retention time. The content of remainder CTH in solid tumor at different time were determined by RP-HPLC accurately, and compared with the retention time of curcumin suspensions for injection （CS）. RESULTS： MCS exosmosed from pinhole while MCTH didn＇t do so. The retention time of CTH was 43.02 h, which was longer than that of CS （P〈 0.01 ）. CONCLUSION： The retention time of CTH is longer in solid tumor and shows its obvious sustained release.