HPLC法测定肠激安提取物中芍药苷的含量

Content Determination of Paeoniflorin in Changji'an Extract by HPLC

目的:建立测定肠激安提取物中芍药苷含量的方法。方法:采用高效液相色谱法。色谱柱为Phenomenex Luna C1(8250m量m在×40..606m4m4,～50μ.6m4)4,0流μ动g范相围为乙内腈与-峰0.1面%积磷积酸分(1值5∶呈85良),好检线测性波关长系为(2r3=00n.m99,9流9)速;为平1均.0加m样L·回m收in-率1,柱为温10为0.3507,℃RS。D结=果2.4:7芍%药(n苷=进9)样。结论:本方法简便、易行、重复性好,可作为肠激安提取物中芍药苷含量的测定方法。

OBJECTIVE： To determine the content of paeoniflorin in Changji＇an extract. METHODS： HPLC method was ad- opted. The determination was performed on a Phenomenex Luna C18（250 mm^4.6 mm, 5 ~tm） column with mobile phase consisted of acetonitrile-0.1% phosphoric acid （15：85） at the flow rate of 1.0 mL-min^-1. The detection wavelength was set at 230 nm and the column temperature was 30 ℃. RESULTS： The linear range was 0.064 4-0.644 0 μg for paeoniflorin （r=0.999 9） with an average recovery of 100.57% （RSD=2.47%, n=9）. CONCLUSION： The method is simple, practical and well reproducible. It can be used as a method for the content determination of Changji＇an extract.