超高效液相色谱法测定人参中人参皂苷Re的含量

Content Determination of Ginsenoside Re in Panax ginseng by UPLC

目的:建立测定人参中人参皂苷Re含量的方法。方法:采用超高效液相色谱法。色谱柱为Waters patened hybrid particle细1.2内39径μg柱·(m5L0-1m范m围×内2.1与m峰m面,1积.7积μm分)值,流呈动良相好为的乙线腈性关-水系((19r=∶801.)9,99检8测);波平长均为加样20回3n收m率。为结9果8.:7人0%参,R皂S苷D=R1e.的23质%(量n=浓6度)。在结0.论53:1本～方法操作便捷、效率高、结果准确,可用于人参的含量测定和质量控制。

OBJECTIVE： To develop the method for the content determination of ginsenoside Re in Panax ginseng. METH- ODS： UPLC method was adopted. The determination was performed on Waters patened hybrid particle column （50mm×2.1mm,1.7μm） with mobile phase consisted of acetonitreile-water （1.9：81） with detection wavelength at 203 nm. RESULTS： The linear range of ginsenoside Re was 0.53141.239 μg.mL^-1（r=0.999 8） with an average recovery of 98.70% （RSD= 1.23% ,n=6）. CONCLUSION： The method is simple, convenient and accurate with high efficacy, and it can be used for the content determination and quality control of P. ginseng.