姜黄素减轻兔单肺通气导致的肺损伤

Curcumin Attenuates Lung Oxidative Injury Induced by One Lung Ventilation in Rabbits

【目的】观察姜黄素对兔单肺通气导致的肺损伤的影响。【方法】12只新西兰白兔随机分为单肺通气组(组Ⅰ)和单肺通气+姜黄素组(组Ⅱ),每组6只。于实验前7 d开始灌胃给药,组Ⅰ给予对照剂,组Ⅱ给予姜黄素40 mg/kg,每天早晚各1次。两组动物均气管切开插入单腔气管导管(ID:3.5 mm)建立右侧单肺通气模型,采用容量通气模式(潮气量12 mL/kg,呼吸频率40 min-1,I∶E=1∶2)并模拟左侧开胸手术。先双肺通气30 min,再单肺通气3 h,恢复双肺通气30 min。监测两组兔血气及气道峰压,计算氧合指数(OI,PaO2/FiO2)。实验结束后处死动物,分别取左、右肺组织观察形态学变化并评分,测定左右侧支气管肺泡灌洗液中蛋白含量并进行中性粒细胞计数,检测肺组织中一氧化氮(NO)、丙二醛(MDA)水平及超氧化物歧化酶(SOD)活力。【结果】与组Ⅰ相比,组Ⅱ氧合指数显著升高(P<0.01)、双侧肺损伤评分、肺组织中NO及MDA水平均降低(P<0.05),SOD活力升高(P<0.05)。同组内左侧肺损伤评分、NO、MDA水平高于右肺(P<0.05),SOD活力低于右肺(P<0.05)。【结论】单肺通气可导致兔肺氧化应激损伤,且非通气侧肺损伤程度较通气侧肺严重;姜黄素预先给药可减轻单肺通气所致的双侧肺损伤。

[Objective] To investigate the effect of curcumin on lung injury induced by one-lung ventilation（OLV） in rabbits. [Methods]Twelve New Zealand white rabbits were randomly allocated into two groups： OLV group （group I ,n = 6）, and OLV＋ curcumin group （group I1 , n = 6）. Rabbits in group I were given 1% sodium carboxymethylcellulose as placebo, while rabbits in group II were received curcumin 40 mg/kg which dissolved in 1% sodium carboxymethylcellulose via gastric tube for seven consecutive days before the operation. All rabbits in two groups were tracheostomized and inserted a ID 3.5 mm trachea tube into the right bronchus to establish one lung ventilation. The rabbits were mechanically ventilated using the volume-control mode （VT 12 ml/ kg, RR40 bpm, I ： E = 1 ： 2 ）after imitating the thoracic surgery. Firstly, they were received two-lung ventilation for 30 rain, then one-lung ventilation for 3h, and finally return to two-lung ventilation for 30 min. Blood gases and airway pressure were monitored and the oxygenation （PaOJFiO2） was calculated before and after the ventilation, respectively. At the end of ventilation, the rabbits were killed and two lungs tissue were sampled respectively for HE stain to assess lung injury degree and get lung damage score. Lungs were excised and bronchoalveolar lavage fluid （BALF） were collected for measurement of protein concentration and neutrophil counts. The contents of nitric oxide （NO）, malondialdehyde （MDA）, and superoxide dismutase （SOD） in the lung tissue were measured. [ Results ] The oxygenation of rabbits in group II was significantly higher（P 〈 0.05 ）, and the right and left lung damage score, BALF protein concentration and neutrophil counts in group ]I were significantly lower （P 〈 0.05）, as compared with group ｜ . The content of MDA and NO in both sides of lung tissue in group 11 were lower than those in group I （P 〈 0.05）, the activity of SOD in group 1I was significantly higher than that in group I （P 〈 0.05）. In each group, the lung damage score, protein concentration in BALF, neutrophilcounts and the contents of NO and MDA of the left （non-ventilated） lung were all higher than those of the right （ventilated） lung （P 〈 0.05）, and the activity of SOD in the left lung was lower than that in the right lung （P 〈 0.05）. [Conclusion] OLV can cause lung oxidative injury in rabbits and the degree of injury in the non-ventilated lung is more serious than that in the ventilated lung. Curcumin pretreatment attenuates OLV induced oxidative injury in rabbits.