摘要
目的:探讨细胞毒性T淋巴细胞相关抗原4(CTLA-4)基因启动子1661位点单核苷酸多态性(SNP)、幽门螺杆菌(Hp)感染与青岛地区人群胃癌的发病率关系。方法:通过聚合酶链反应-限制性片段长度多态法分析来自青岛地区人群胃癌、萎缩性胃炎及对照组患者中CTLA-4-1661A/G基因多态性的分布。Hp检测采用病理学诊断。结果:1)胃癌组启动子1661位AG+GG基因型频率显著高于对照组(P=0.01),携带AG+GG基因型的个体罹患胃癌的风险提高2.63倍。2)以AA基因型并HP阴性者为对照,AG+GG基因型并Hp阴性个体、AA基因型并Hp阳性、AG+GG基因型并Hp阳性个体胃癌患病风险增高,其中AG+GG基因型并Hp阳性个体的患病风险显著增高,为对照组的4.04倍(P=0.008)。结论:CTAL-4-1661基因多态性与胃癌的遗传易感性相关。
Objective: To analyze the relationship between promoter 1661 site in CTLA-4 gene polymorphism and infection of Helicobacter in Qingdao area and susceptibility to gastric cancer genesis.Methods: Polymerase chain restriction fragment length polymorphism and direct sequencing were performed to analyze the genotype of the A/G polymorphism in 1661 site region,and Hpwas detected by pathological diagnosis.Results: 1)The frequency of AG+GG genotype in 1661site was higher in the gastric cancer group compared to the control group(P=0.01),moreover,individuals with the CTLA-4-1661AG+GG genotype rose to 2.63 fold risk for gastric cancer.2)Compared with CTLA-4 the AA genotype and the H.pylori negative individuals,there was an increased risk of developing gastric cancer for those carrying AG+GG genotype and H.pylori positive individuals.Conclusion: There was a relationship between CTLA-4-1661 A/G polymorphism and susceptibility to gastric cancer.
出处
《中国现代普通外科进展》
CAS
2011年第12期937-940,共4页
Chinese Journal of Current Advances in General Surgery