摘要
目的:探讨细胞毒性T淋巴细胞相关抗原4(CTLA-4)基因启动子1661位点单核苷酸多态性(SNP)、幽门螺杆菌(Hp)感染与青岛地区人群胃癌的发病率关系。方法:通过聚合酶链反应-限制性片段长度多态法分析来自青岛地区人群胃癌、萎缩性胃炎及对照组患者中CTLA-4-1661A/G基因多态性的分布。Hp检测采用病理学诊断。结果:1)胃癌组启动子1661位AG+GG基因型频率显著高于对照组(P=0.01),携带AG+GG基因型的个体罹患胃癌的风险提高2.63倍。2)以AA基因型并HP阴性者为对照,AG+GG基因型并Hp阴性个体、AA基因型并Hp阳性、AG+GG基因型并Hp阳性个体胃癌患病风险增高,其中AG+GG基因型并Hp阳性个体的患病风险显著增高,为对照组的4.04倍(P=0.008)。结论:CTAL-4-1661基因多态性与胃癌的遗传易感性相关。
Objective： To analyze the relationship between promoter 1661 site in CTLA-4 gene polymorphism and infection of Helicobacter in Qingdao area and susceptibility to gastric cancer genesis.Methods： Polymerase chain restriction fragment length polymorphism and direct sequencing were performed to analyze the genotype of the A/G polymorphism in 1661 site region,and Hpwas detected by pathological diagnosis.Results： 1）The frequency of AG＋GG genotype in 1661site was higher in the gastric cancer group compared to the control group（P=0.01）,moreover,individuals with the CTLA-4-1661AG＋GG genotype rose to 2.63 fold risk for gastric cancer.2）Compared with CTLA-4 the AA genotype and the H.pylori negative individuals,there was an increased risk of developing gastric cancer for those carrying AG＋GG genotype and H.pylori positive individuals.Conclusion： There was a relationship between CTLA-4-1661 A/G polymorphism and susceptibility to gastric cancer.
出处
《中国现代普通外科进展》
CAS
2011年第12期937-940,共4页
Chinese Journal of Current Advances in General Surgery
