KIF14在胃癌中的表达及其意义

Expression and significance of KIF14 in gastric cancer

目的:探讨KIF14在胃癌中的表达及其意义。方法:应用实时定量聚合酶链反应技术检测30例胃癌中KIF14 mRNA的表达,应用免疫组织化学方法检测89例胃癌、正常黏膜及35例转移淋巴结中KIF14蛋白的表达。结果:KIF14 mRNA在66.7%的胃癌组织中高表达;KIF14蛋白在胃癌组织中的表达率为62.9%,高于正常黏膜(6.74%,P<0.05),且与TNM分期(P<0.01)、浸润深度(P<0.05)、淋巴结转移(P<0.05)、远处转移(P<0.05)及Ki67相关。转移淋巴结中KIF14蛋白表达率(82.9%)高于原发癌和正常黏膜(P<0.01)。KIF14蛋白阳性患者总体生存率和无病生存率均显著降低,KIF14是影响总体生存(P<0.001)和无病生存(P<0.001)的独立预后因素。结论:KIF14在胃癌发生发展中起重要作用,可能是新的预后指标。

Objective： To explore the expressiom of KIF14 in gastric cancer and its significance.Methods： KIF14 mRNA expression was detected in 35 cases of gastric cancer and the corresponding para-cancerous tissue by real-time polymerase chain reaction（PCR）;KIF14 protein expression was detected by immunohistochemistry on tissue microarray,which containing 89 samples of primary gastric cancer and the corresponding para-cancerous tissue,and 30 paired lymph node metastases（LNM）.Results： KIF14 mRNA was upregulated more than 2-fold in 66.7% patients with gastric cancer.KIF14 protein was upregulated in gastric cancer and correlated with clinical stage,invasion depth,LNM,distant metastasis and Ki67 index.Immnoreactivity of KIF14 was significantly higher in LNM than in primary gastric cancer and normal mucosa.The expressiom of KIF14 mRNA and protein in gastric cancer is coincidence.The patients positive for KIF14 had lower overall survival（OS） and disease-free survival（DFS） than those negative for KIF14.KIF14 was an independent prognostic factor for OS（P〈0.01） and DFS（P〈0.01）.Conclusion： KIF14 may play an important role in gastric cancer tumorigenesis and progression and could serve as a predictor in prognosis in gastric cancer.