雄性大鼠脊髓损伤对生殖系细菌感染及IL-1、TNF-α、SOD表达的影响

Spinal cord injury in male rats can impact bacterial infection of reproductive system,and IL-1,TNF-α and SOD expressions

目的:了解脊髓损伤后雄性大鼠前列腺、附睾、睾丸细菌感染及IL-1、TNF-α、SOD蛋白的表达情况。方法:80只SD大鼠采用随机数字法分为对照组(n=40)和实验组(n=40),采用改良Allen法建立脊髓损伤动物模型,分别喂养1、2、4、8、16 d,2组均于每个时间点摘取8只大鼠的前列腺、附睾、睾丸组织进行细菌培养和免疫组织化学SABC法检测白介素-1(Interleukin-1,IL-1)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、超氧化物歧化酶(Superoxide dismutase,SOD)的表达。结果:与对照组相比,实验组前列腺、附睾细菌培养阳性数显著提高(P<0.05),大肠杆菌的检出率高于其他细菌;睾丸IL-1、TNF-α表达明显增多(P<0.05),实验组间以术后第4天表达水平最高(P<0.05);SOD表达无明显差异(P>0.05)。结论:脊髓损伤后大鼠前列腺、附睾易受到以大肠杆菌为主的细菌感染,睾丸炎性反应增强,生殖系抗氧化反应可能很弱。

Objective：To study the expressions of IL-1,TNF-α,SOD and bacterial infection in male rats＇ reproductive system after spinal cord injury.Methods：80 SD rats were divided into control group（40 rats） and experimental group（40 rats） randomly.Animal models of spinal cord injury were established by ＇Allen＇ method.Both groups of rats were fed for 1 day,2 days,4 days,8 days,and 16 days respectively.Then 8 rats＇ prostates,testes and epididymises in each group were extirpated for bacterial culture and immunohistochemistry by SABC to detect the expressions of proteins（IL-1,TNF-α,SOD） at each time point.Results：Compared with control group,the risk of E.coli infection in prostate and epididymis was increased significantly in the experimental group（P0.05）;the expressions of testicular IL-1 and TNF-α were increased（P0.05） and the expression level was the highest in the fourth days after surgery（P0.05）.SOD expression showed no significant difference in the whole reproductive system（P0.05）.Conclusion：Prostate and epididymis of the rat after spinal cord injury are easily infected by bacteria（E.coli）;inflammatory response was enhanced in testicular,and the antioxidant activity of reproductive system may be very weak.