提前获取早熟凝集染色体环快速估算受照剂量的可行性研究

Feasibility of rapidly estimating biological dose under shortened incubation of premature chromosome condensation rings

目的:缩短培养时间,拟合γ射线诱发离体人外周血淋巴细胞早熟凝集染色体环(premature condensation chromosomerings,PCC-R)的剂量-效应曲线,探讨快速估算生物剂量的可行性。方法:^(60)Coγ射线照射离体人外周血,剂量为0、1、3、6、10、15、20 Gy,照后分别培养44和48 h,收获前1 h加入冈田酸,分别计数PCC-R频率,拟合受照44与48 h的剂量-效应曲线并进行比较。结果:培养44 h较48 h获得的分析细胞数略少,PCC-R率略低于48 h结果(各个剂量点间的差异均无统计学意义)。外周血淋巴细胞PCC-R率随照射剂量的增加而增加,0～15 Gy剂量范围内二者之间满足直线方程Y=-0.015 2+0.062 5D(R^2=0.983 9)。结论:缩短培养时间至44 h,PCC-R仍然可以用于15 Gy以内的辐射事故的剂量估算。

OBJECTIVE：To explore the feasibility of rapidly estimating biological dose under shortened incubation time and establish dose-effect curve of premature chromosome condensation rings（PCC-R） in human lymphocytes induced byγ-rays.METHODS：Human peripheral blood was irradiated in vitro by ^（60）Coγ-rays at 0,1,3,6,10,15 and 20 Gy.PCC-R was counted after 44 h and 48 h incubation with Okadaic acid added 1 h before harvest.Both 44 h and 48 h dose-effect curves were established and compared.RESULTS：Cells obtained from 44 h incubation was slightly decreased,and PCC rings rate was slightly lowered compared with 48 h incubation（P〉0.05）.PCC rings rate increased with dose and demonstrated a perfect linear relationship（0-15 Gy）,with a linear equation Y=-0.015 2＋0.0625D（R2=0.983 9）. CONCLUSION：After shortening the incubation time to 44 h,PCC rings could still be used in dose estimation of high dose radiation incidents within 15 Gy.