hprt基因突变对宫颈癌放疗损伤的评估

Assessment of radiotherapy damage using hprt gene mutation in cervical cancer patients

目的:探讨hprt基因突变对宫颈癌放疗损伤的评估作用。方法:取15例宫颈鳞癌放疗患者放疗前及放疗累积剂量为10～60Gy时的外周静脉血,采用多核细胞法检测hprt基因突变率。结果:在照射累积剂量为30、40和50Gy时,hprt基因突变率在照射后升高,与照射前相比,差别有统计学意义(P<0.05)。当累积剂量达60 Gy时,hprt基因突变率虽然较照射前升高,但差别无统计学意义(P>0.05)。在0～40 Gy,hprt基因突变率基本呈线形上升,且突变率与累积剂量间呈现较好的剂量-效应关系(r=0.900,P<0.05)。回归方程为y=0.459+0.014x。结论:在累积剂量0～40 Gy范围内,hprt基因突变率在放疗后明显升高,呈现良好的剂量-效应关系,可用于评价放疗造成的损伤,是具有应用潜质的生物剂量计。

OBJECTIVE：To assess the radiotherapy damage using hprt gene mutation in cervical cancer patients.METHOD：Blood samples were collected from 15 cervical cancer patients exposed to radiation at the accumulated dose of 0-60 Gy.hprt gene mutation was studied by multinucleated cell assay.RESULT：Mutation frequency of hprt gene increased after radiation.The frequency increased significantly at dose 30,40 and 50 Gy compared to 0 Gy（P〈0.05）.At the accumulated dose of 60 Gy,the mutation frequency was also higher than 0 Gy,but not statistically significant.The increase of hprt gene mutation frequency was linear from 0-40 Gy.There was a good dose-response effect between mutation frequency of hprt gene and accumulated dose（r=0.900,P〈0.05）.Analysis showed a linear dose-response with an equation of y=0.459＋0.014x（r=0.900,P〈0.05）.CONCLUSION：Mutation frequency of hprt gene increased after radiation from 0-40 Gy.There was a good dose-response relationship between mutation frequency and accumulated dose.Mutation frequency of hprt gene could be a valuable biodosimeter.