长期培养大鼠海马脑片CAl区基质细胞诱导因子和CXCR4蛋白的免疫活性变化

Immunoreactivity changes of SDF-lα and CXCR4 proteins in CA1 area of the long-term organotypic hippocampal slice culture in rat

目的：研究基质细胞诱导因子一lα（SDF-1α）和CXCR4蛋白在长期培养的海马脑片CAl区的表达变化。方法：运用免疫组织化学法检测SDF-1cc和CXCR4在大鼠海马脑片CAl区的表达变化。结果：培养1-5周海马脑片CAl区均有SDF-1α和CXCR4的蛋白表达，阳性细胞的胞质呈棕黄色染色。培养1周时，CXCR4蛋白的表达水平较高，随着培养时间的延长，表达呈下降的趋势，培养至5周的海马脑片CAl区CXCR4蛋白表达水平和培养1周相比差异有统计学意义。培养1周的海马脑片CAl区SDF-1a的表达也处于较高水平，培养至2-3周时有所下降，但与培养1周相比无差异，培养至4-5周时其表达则下降。结论：SDF-1α和CXCR4蛋白在大鼠海马脑片CAl的表达变化具有时空性差异，提示SDF-1／CXCR4生物轴参与了海马脑片在体外的生长，并可能介导了CAl区神经元细胞的分化。

Objective： To investigate the expressional changes of stromal cellderived factor-lα（SDF-la） and CXCR4 proteins in CA] area during the long-term hippocampal organotypic tissue culture （HOTC） in rats. Methods： Immuno histochemical analysis was used to detect SDF-1α and CXCR4 expression in CA1 area of hippocampus slice. Results： The immunohistochemical reactions of SDF-1 α and CXCR4 were positive in CA1 area of hippocampal slice with the cell membrane and cytoplasm stained from cultured 1 week to cultured 5 weeks. The expression of CXCR4 was higher at 1 week when it was compared with the cultured 5 weeks, and then its expression was descending trend. The expression of SDF-I~ protein was high at cultured 1 week, while its expression was decreased gradually from cultured 2 weeks to 5 weeks. Conclusion： The protein expressions of SDF-lα and CXCR4 in longtime HOTCs of rats are of spatialtemporal dependent features, which implys that the SDF-1/CXCR4 axis take part in the development of H OTC, and may be relat- ed to cell differentiation of CAl area.