核转录因子E2F-1、P27在大肠癌中的表达及意义

Expression and significance of nuclear transcription factor E2F-1 and P27 in colorectal adenocarcinoma

[目的]探讨大肠癌组织E2F-1和P27表达的意义。[方法]用免疫组化SP法检测78例大肠癌、20例正常大肠黏膜、30例大肠腺瘤E2F-1和P27的表达情况,并分析二者在大肠癌发生中的相关性。[结果]E2F-1表达阳性率在大肠癌(55.13%)显著高于正常大肠黏膜(0%)及大肠腺瘤(6.67%)(P<0.05);E2F-1表达与大肠癌的病理分级、淋巴结转移和临床分期相关(P<0.05)。P27在正常大肠黏膜的阳性率(100%)显著高于大肠腺瘤(53.33%)和大肠癌(15.38%)(P<0.05)。P27表达与大肠癌的病理分级、淋巴结转移和临床分期相关(P<0.05)。大肠癌中E2F-1与P27表达呈负相关(r=-0.393,P<0.01)。[结论]E2F-1和P27表达与大肠癌发生关系密切,可作为大肠癌发生、发展的生物学标记物。

[ Objective] To study the relationship of E2F - 1 and P27 expression to occurence of colorectal adenocarcinoma and its clinical significance. [Methods~ The expressions of E2F- 1 and P27 were detected in 78 colorectal adenocareino- mas, 30 colorectal adenomas and 20 nomlal coloreetal mucosa by immunohistochemical SP method. [ Results] The positive expression rate of E2F - 1 （ 55.13% ） in the carcinoma was higher than those in colorectal adenoma （6.67%） and mueo- sa （0%） （P 〈0.05 ）. The expression of E2F- 1 in coloreetal carcinoma was positively related to pathological grade, lymph node metastasis and clinical stage （P 〈 0.05 ）. 1927 positive rate in normal colorectal mucosa （100%） was higher than those of colorectal adenoma （53.33%）and colorectal carcinoma （ 15.38% ）. The expression of P27 in colorectal car- cinoma was negatively related to pathological grade, lymph node metastasis and clinical stage （ P 〈 0.05 ）. In colorectal carcinoma, E2F - 1 and P27 expressions were negatively correlated （r = ＂0. 393, P 〈 0.01 ）. [ Conclusion] E2F - 1 and P27 is closely related to the colorectal adenocarcinoma occurrence, and both of them may have possibilities as biological markers in colorectal adenocarcinoma.