S100A4基因在不同骨肉瘤细胞和组织中的表达

Expression of S100A4 gene on different osteosarcoma cell lines and tissues

[目的]检测S100A4基因在人骨肉瘤细胞系MG-63、U-2OS以及骨肉瘤组织中的表达情况并分析其临床意义。[方法]应用Real-time PCR方法检测S100A4基因在人骨肉瘤细胞MG-63及U-2OS中的表达,用免疫组织化学SP法检测骨肉瘤S100A4、CD44V6蛋白表达,并分析二者的关系。[结果]人骨肉瘤细胞系MG-63及U-2OS中均有S100A4基因表达;S100A4蛋白在骨肉瘤组织中表达的阳性率是69.7%,表达位置在骨肉瘤细胞的细胞浆,CD44V6蛋白在骨肉瘤组织中表达的阳性率是60.6%,表达位置在骨肉瘤和骨软骨瘤细胞均为细胞膜和近膜胞浆,二者的表达呈正相关(r=0.413,P<0.05)。[结论]S100A4基因在不同的骨肉瘤组织及其细胞系均有较高水平表达,其表达可能在侵袭和转移中发挥作用。

[ Objective] To detect the expression of S100A4 gene in human osteosarcoma MG-63 and U -2OS cell lines and the expressions of S100A4 and CD44V6 in osteosarcoma tissues, and then analyze its clinical significance. [ Meth- ods ] The expressions of S100A4 gene in human osteosarcoma MG -63 and U -2OS cell lines were detected by real -time PCR. The protein expressions of S100A4 and CD44V6 were detected by immunohistochemistry SP method, and anlyzed the relationship between both things. [ Results~ The result of real - time PCR showed that human osteosarcoma cell lines of MG- 63 and U -2OS had the expression of S100A4 gene. The positive rate of S100A4 protein expression in osteosarcoma tissues was 69.7%, and the expression located in cytoplasm. The positive rate of CI）44V6 protein expression in osteosarco- ma tissues was 60.6% , and the expression of CD44V6 located at cell membrane and cytoplasm near the cell membrane of osteosarcoma cells and osteochondroma cells. The expression of S100A4 was positively related to that of CD44V6 in osteo- sarcoma tissues （r = 0. 413, P 〈 0.05 ）. [ Conclusion] S100A4 gene expresses in human osteosarcoma cells in vitro and in vivo. S100A4 might play a role in invasion and metastasis of osteosarcoma.