摘要
对青海省祁连山永久冻土区天然气水合物DK-2钻孔的11件样品进行分析,通过微生物群落分析来探寻水合物层样品与非水合物层样品的差别。在11件样品中均发现了细菌16S rDNA,未检测到海洋天然气水合物地区常见的古菌16S rDNA、mcrA(Ⅰ,Ⅱ)、pmoA、mmoX和mxaF。分析得到的细菌16S rDNA分属5个门,包括变形杆菌门、放线菌门、拟杆菌门、厚壁菌门和异常球菌-栖热菌门,随着样品深度的增加,细菌多样性有降低的趋势。对非水合物层样品DK2-19和水合物层样品DK2-25进行细菌系统发育树分析,发现这2个样品群落结构相差较大。水合物层样品与非水合物层样品细菌群落对比后发现,水合物层样品中γ-变形杆菌的比例低于非水合物层样品中γ-变形杆菌的比例,而Arthrobacter属多发现于非水合物层的样品中。
Eleven cores from DK-2 gas hydrate borehole in Qilian Mountain permafrost, located in the north of the Tibetan Plateau, were investigated to find out the difference between gas hydrate samples and non-gas hydrate samples by microbial conmlunity analysis. Of all the genes often found in marine gas hydrate samples, i.e., bacterial and archaeal 16S rDNA, mcrA （ I , lI ）, pmoA, mmoX and mxaF, bacterial 16S rDNA could be observed in all of the eleven samples, resulting in the detection of a great quantity of bacterial se- quences belonging to 5 phylums, i.e., Proteobaeteria, Actinobaeteria, Baetemides, Firmicutes, and Deinoeoecus-Thermus. The bacteria diversity decreases with the increasing depth. A study of gas hydrate sample DK2-19 and non-gas hydrate sample DK2-25 selected for phylogenetie analysis and diversity comparison shows that the two samples are remarkably different from each other. A comparison between gas hydrate samples and non-gas hydrate samples show that the percentage of y-Proteobaeteria in gas hydrate samples is lower than that in non-gas hydrate samples, and Arthrobaeter could be found mainly in non-gas hydrate samples.
出处
《地质通报》
CAS
CSCD
北大核心
2011年第12期1874-1882,共9页
Geological Bulletin of China
基金
中国地质调查局地质调查项目《青藏高原冻土带天然气水合物调查评价》(编号:1212010818055)
