银杏叶片长期给药后对大鼠灌服氯沙坦的药代动力学影响

Effect of Ginkgo biloba talbes on the pharmacokinetics of intragastrically administered losartan in rats

目的:研究银杏叶提取物(GBE)制剂银杏叶片长期给药后对大鼠灌服氯沙坦(Los)的药代动力学影响。方法:SD大鼠随机分为Los单用组,GBE+Los联合用药组。试验第1天起,联合用药组大鼠灌胃给予GBE 100mg/kg,共14d。单用组大鼠灌胃给予等量纯净水。第15天,单用药组大鼠灌胃给予Los 10mg/kg,GBE+Los联合给药组大鼠同时灌胃给予GBE 100mg/kg和Los 10mg/kg。于Los给药后0.5、1、2、3、4、6、8、10、12、24h从大鼠眼眦静脉丛取血。HPLC法测定血浆中Los及其活性代谢产物EXP3174的浓度,计算其主要药代动力学参数,并进行统计学分析。结果:合用GBE后,Los的各药代动力学参数差异无统计学意义。EXP3174的tmax显著缩短(P<0.05),Cmax、AUC0-8和AUC0-∞显著增加(P<0.01),CL显著降低(P<0.05),t1/2、MRT变化无统计学差异。结论:GBE能够影响EXP3174的体内代谢,但对Los的药代动力学过程影响没有统计学意义。

To investigate the possible effect of ginkgo biloba tablets which were prepared by Ginkgo biloba extracts （GBE） on the pharmacokinetics of losartan by intragastrically administering in rats. METHODS： SD rats were randomly divided into two groups （losartan group and GBE＋losartan group）. Rats in GBE losartan group were administered with GBE 100 mg/kg from day 1 to 14. On day 15, Rats in the losatan group were administered with losar- tan 10 mg/kg, and Rats in the GBElosartan group were added with GBE 100 mg/kg. Blood samples were collected from the angulus oculi veins at 0. 5,1,2, 3,4, 6,8, 10, 12, 24 h. The blood plasma concentration of losartan and EXP3174 were determined by HPLC. Main pharmacokinetic parameters were calculated and cornpared by statistical analysis. RESULTS.After coadministrating with GBE, there were no significantly different pharmacokinetic parameters of losartan were found. However, the t_max of EXP3174 was markedly decreased （P〈0.05）. The C AUC0-sh, AUC0of EXP3174 were significantly increase （P〈0.01）. And the CL of EXP3174 was markedly decreased （P〈0.01）. There was no significantly changed of tl/2 and MRT about EXP3174. CONCLUSION： GBE can affect the metabolism of no significantly different ics of losartan. EXP3174, which were on the pharmacokinetics of losartan.